Plasmid carrying mcr-9 from an extensively drug-resistant NDM-1-producing Klebsiella quasipneumoniae subsp. quasipneumoniae clinical isolate.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
D FacconeA Petroni

Abstract

The extensively drug-resistant NDM-1-producing Klebsiella quasipneumoniae subsp. quasipneumoniae M17277 (Kqsq-M17277) was only susceptible to colistin and fosfomycin. Whole genome sequencing of Kqsq-M17277 was performed through Illumina (short reads) and Oxford Nanopore Technologies (long reads). Hybrid assembly of short and long reads rendered circular contigs of the Kqsq-M17277 chromosome and three plasmids of 477,340 (p17277A_477), 138,998 (p17277C_138, which harbored blaNDM-1) and 123,307 bp (p17277B_123). In silico analysis showed that p17277A_477 was not previously described, belonged to the IncHI2 incompatibility group and contained 51 complete or partial (≥300 bp) insertion sequences and Tn3 family transposons, which encompassed, as a whole, 14% of the p17277A_477 sequence. In silico search for antimicrobial resistance genes revealed that p17277A_477 harbored the recently described plasmid-encoded mcr-9 gene (colistin resistance), besides other resistance genes. This is the first worldwide report of a K. quasipneumoniae harboring mcr-9 and blaNDM-1 and the first report of mcr-9 in Latin America. This gene was embedded in a genetic structure, i.e., IS903B-like/mcr-9/wbuC/IS26, that was found in 71% of the mcr-9-harboring...Continue Reading

Citations

Jun 6, 2020·Microbiology Resource Announcements·Clement K M TsuiAndres Perez-Lopez
Dec 17, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Aunyarat KhanawapeeShigeyuki Hamada
Mar 25, 2021·Mobile DNA·Alessandro VaraniMichael Chandler
Apr 3, 2021·International Journal of Antimicrobial Agents·Xiaoming WangLiping Wang
Apr 30, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Nenad MacesicAnton Y Peleg
May 25, 2021·Frontiers in Public Health·Silvia Adriana Mayer LentzAndreza Francisco Martins
Jul 3, 2021·Diseases·Ozioma Forstinus NwaborSarunyou Chusri
Aug 23, 2021·International Journal of Antimicrobial Agents·J Rodríguez-SantiagoU Garza-Ramos
Sep 11, 2021·Frontiers in Cellular and Infection Microbiology·Barbara GhiglioneMilena Dropa

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