PMID: 6171811Oct 1, 1981Paper

Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript

Proceedings of the National Academy of Sciences of the United States of America
J Tomizawa, T Itoh

Abstract

Mutants of plasmid pNT7 that can coexist with plasmid pMB9 in growing bacteria have been isolated. These mutants show altered incompatibility properties and increased copy numbers. Each mutant has a single base change at or near the center of one of the three palindromes in the region that specifies two RNA species: a larger one (primer transcripts) that provides a primer for DNA replication and a smaller one (RNA I) that is the incompatibility-specific inhibitor of primer formation. In vitro transcription studies show that the single base changes affect both the ability of RNA I to inhibit primer formation and the sensitivity of primer formation to inhibition by RNA I. RNA I hybridizes to the primer transcript, and the rate of hybridization is reduced by the single base changes. Based on analyses of inhibition of in vitro primer formation by RNA I and of in vivo properties of the mutant plasmids, we conclude that incompatibility between two plasmids can be attributed to inhibition of primer formation on one of the plasmids by the RNA I of the other. Inhibition of primer formation by RNA I appears to be the mechanism that determines the copy number of pNT7 and its derivatives.

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·D R MoserJ L Campbell
Nov 1, 1988·Molecular & General Genetics : MGG·Y TajimaT Itoh
Feb 1, 1988·Molecular & General Genetics : MGG·J MorlonC Lazdunski
Jan 1, 1983·Molecular & General Genetics : MGG·P Polaczek, Z Cieśla
Jan 1, 1984·Molecular & General Genetics : MGG·T Ogawa, T Okazaki
Jan 1, 1982·Molecular & General Genetics : MGG·T Som, J Tomizawa
Jan 1, 1982·Molecular & General Genetics : MGG·J Light, S Molin
May 1, 1988·Molecular & General Genetics : MGG·T Horii, T Itoh
Jan 1, 1989·Molecular & General Genetics : MGG·H YasuedaT Itoh
Jan 1, 1983·Molecular & General Genetics : MGG·B C Kline, J Trawick
Aug 1, 1986·Molecular & General Genetics : MGG·S J Projan, R P Novick
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