Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.

Journal of Bacteriology
M J Gasson

Abstract

The production and regeneration of bacterial protoplasts promoted the loss of three different plasmid-specified traits in Streptococcus lactis subsp. diacetylactis strains. The loss of five different plasmids, including small multicopy molecules, was readily detected in Streptococcus lactis 712 by screening lysates of random protoplast regenerants on agarose gels. In this strain sequential rounds of protoplast regeneration were used to produce a plasmid-free strain and derivatives carrying only single molecules from the plasmid complement. During these experiments a 33-megadalton plasmid, pLP712, was found to encode genes for lactose and protein utilization. Only this plasmid was required for normal growth and acid production in milk; the remaining four plasmids appeared to be cryptic. Lactose-defective derivatives of a strain carrying only pLP712 were readily isolated. Although these derivatives included instances of plasmid loss, deletions of pLP712 were frequently found. Many different deleted derivatives of pLP712, including some in which the lactose or protein utilization determinant or both were lost, were isolated. The molecular instability of pLP712 largely accounted for previous observations of plasmid complements in S...Continue Reading

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Citations

May 1, 1994·Molecular Microbiology·J J GodonM J Gasson
Jan 1, 1993·Journal of Dairy Science·D A Romero, T R Klaenhammer
Jun 1, 1993·The Journal of Applied Bacteriology·J M WellsR W Le Page
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Jul 10, 2001·Letters in Applied Microbiology·N CorneauE Emond
Jun 19, 2001·Biotechnology and Bioengineering·C R MelchiorsenJ Villadsen
Aug 10, 2001·Microbiology·A BreünerK Hammer
Apr 27, 2002·Applied and Environmental Microbiology·Nathalie CampoPascal Le Bourgeois
Apr 26, 2002·Environmental Microbiology·Ulrika Andersson, Peter Rådström
May 3, 2002·Molecular Microbiology·Stephen McGrathDouwe van Sinderen
Oct 9, 2002·Molecular Microbiology·Carine MercierSaulius Kulakauskas
Aug 30, 2002·Applied and Environmental Microbiology·Brian J KoebmannPeter R Jensen
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Mar 7, 2003·Applied and Environmental Microbiology·Robèr KempermanJan Kok
Mar 14, 2003·Microbiology·Carine HuardMarie-Pierre Chapot-Chartier
Aug 19, 2003·Journal of Bacteriology·Pekka VarmanenHanne Ingmer
Jul 4, 2003·Applied and Environmental Microbiology·J Reunanen, P E J Saris
Dec 9, 2003·Applied and Environmental Microbiology·Laura E MacConaillDouwe Van Sinderen

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