Abstract
Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly repeated sequences were rare. In contrast, about half of the deletions in the addB72 mutant resulted from recombination at direct repeats of 5 bp or more. The sequences at or near the left deletion endpoints showed striking similarities in the three strains. (1) 5'-T-T-T-3', or the complement 5'-A-A-A-3', was frequently located at these sites. (2) 5'-T-G-T-A-3' was found close to most of these termini. (3) Nearly all left termini occurred in a region rich in hyphenated dyad symmetry, which includes the penP transcription/translation regulatory sequences. It is assumed that DNA secondary structures, together with a sequence preference, specify the majority of the left deletion termini, which we speculate to be target sites for topoisomerase I. The right termini of deletions in the wild-type and addB72 mutant were frequently located close to a loose octanucleotide consensus sequence: 5'-G/C-G/C-G/C-G-A/T-A/T-A/T-A/G-3...Continue Reading
References
Aug 1, 1979·European Journal of Biochemistry·M F ShemyakinA V Chestukhin
Oct 3, 1979·Molecular & General Genetics : MGG·G RapoportR Dedonder
Feb 24, 1989·Cell·G J Pruss, K Drlica
Jan 1, 1989·Annals of the New York Academy of Sciences·P H DuesbergD Goodrich
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Mar 25, 1988·Cell·D BrunierS D Ehrlich
May 6, 1988·Cell·H Y WuL F Liu
Oct 5, 1985·Journal of Molecular Biology·K Kirkegaard, J C Wang
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B Michel, S D Ehrlich
Jan 1, 1988·Trends in Genetics : TIG·S C West
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H te RieleS D Ehrlich
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P E Love, R E Yasbin
Dec 1, 1986·Journal of Bacteriology·T HimenoS Aiba
Mar 1, 1987·Plasmid·A A PeijnenburgG Venema
Jul 1, 1988·Journal of Bacteriology·J C AlonsoG Lüder
Jun 1, 1988·Molecular & General Genetics : MGG·B P PeetersG Venema
Jul 1, 1988·Plasmid·A C PeijnenburgG Venema
Jan 1, 1987·Annual Review of Biochemistry·M M Cox, I R Lehman
Jan 1, 1985·Annual Review of Biochemistry·G C Walker
Jan 1, 1974·Mutation Research·J DolyC Anagnostopoulos
May 1, 1972·Mutation Research·S Bron, G Venema
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P LopezS A Lacks
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·K KirkegaardJ C Wang
Mar 1, 1981·European Journal of Biochemistry·J DolyC Anagnostopoulos
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·H IkedaT Matsumoto
Apr 5, 1982·Journal of Molecular Biology·K MizuuchiM Gellert
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jun 1, 1982·Cell·A M AlbertiniJ H Miller
Jan 1, 1982·Molecular & General Genetics : MGG·I M JonesS D Ehrlich
Nov 1, 1983·Gene·W M de VosG Venema
Jan 1, 1982·Molecular & General Genetics : MGG·W M de Vos, G Venema
Apr 1, 1987·Genetics·P Anderson
Citations
Apr 1, 1991·Molecular & General Genetics : MGG·S BronB P Peeters
Aug 30, 1995·Molecular & General Genetics : MGG·R MeimaS Bron
Mar 26, 2010·Folia Microbiologica·H Ouled-HaddarH M Saeed
Sep 1, 1991·Research in Microbiology·S D EhrlichA Gruss
Sep 1, 1991·Research in Microbiology·S BronP Haima
Jun 10, 1998·Nucleic Acids Research·R MeimaS de Jong
Feb 1, 1997·Journal of Bacteriology·R MeimaS Bron