PMID: 15222798Jun 30, 2004Paper

Plasmid DNA breakage by decay of DNA-associated Auger electron emitters: approaches to analysis of experimental data

Radiation Research
Pavel N LobachevskyRoger F Martin

Abstract

Plasmid DNA is a popular substrate for the assay of DNA strand breakage by a variety of agents. The use of the plasmid assay relies on the assumption that individual damaging events occur at random, which allows the application of Poisson statistics. This assumption is not valid in the case of damage arising from decay of DNA-associated Auger electron emitters, since a single decay event can generate a few breaks in the same DNA strand, which is indistinguishable from a single break in the assay. The consequent analytical difficulties are overcome by considering relaxation events rather than single-strand breaks, and linearization events rather than double-strand breaks. A further consideration is that apart from damage at the site of DNA-associated decay, which is the principal interest of the analysis, some DNA damage also arises from the radiation field created by all decay events. These two components of damage are referred to as internal and external breakage, respectively, and they can be separated in the analysis since their contribution depends on the experimental conditions. The DNA-binding ligand Hoechst 33258 labeled with 125I was used in our experiments to study breakage in pBR322 plasmid DNA arising from the decay ...Continue Reading

References

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Citations

Aug 10, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Franz BucheggerAngelika Bischof Delaloye
Aug 31, 2010·The Journal of Physical Chemistry. B·Yong JiangPiotr E Marszalek
Mar 15, 2005·International Journal of Radiation Biology·Pavel N Lobachevsky, Roger F Martin
Dec 9, 2008·International Journal of Radiation Biology·Michel TerrissolEkkehard Pomplun
Dec 9, 2008·International Journal of Radiation Biology·Pavel N LobachevskyRoger F Martin
Dec 9, 2008·International Journal of Radiation Biology·Roger W Howell
Feb 3, 2009·Biophysical Journal·Yong JiangPiotr E Marszalek
Jun 27, 2012·International Journal of Radiation Biology·Pichumani BalagurumoorthyAmin I Kassis
May 8, 2007·Biophysical Journal·Yong JiangPiotr E Marszalek
Feb 24, 2016·International Journal of Radiation Biology·Pavel LobachevskyRoger F Martin
Aug 3, 2006·Radiation Research·Pichumani BalagurumoorthyAmin I Kassis
Jul 28, 2005·Radiation Protection Dosimetry·G GrafströmS-E Strand
Nov 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Agnieszka MorgenrothSven N Reske
Jan 19, 2021·Nuclear Medicine and Biology·Dmitry FilosofovValery Radchenko

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