Plasmid DNA encoding caprine interferon gamma inhibits antibody response to caprine arthritis-encephalitis virus (CAEV) surface protein encoded by a co-administered plasmid expressing CAEV env and tat genes

Vaccine
W P CheeversI Hötzel

Abstract

This study characterized immune responses of Saanen goats co-immunized with pUC18 based plasmids expressing caprine arthritis-encephalitis virus (CAEV) rev-env (pENV) or tat-rev-env (pTAT-ENV) and a second plasmid encoding caprine interferon gamma (IFNgamma). A previous study reported that immunization with pENV or pTAT-ENV induces IgG2 biased antibody responses to plasmid encoded CAEV surface envelope protein (SU). We show here that regional lymph nodes (LN) of pENV and pTAT-ENV immunized goats contain a dominant subset of SU activated IFNgamma+ Th1 lymphocytes. Co-immunization with pENV or pTAT-ENV and a caprine IFNgamma cDNA expression plasmid (pcIFNgamma) did not potentiate activation of SU responsive Th1 lymphocytes. However, the antibody response to SU encoded by pTAT-ENV was inhibited by co-immunization with pcIFNgamma. Results indicate that synergistic effects of CAEV Tat and IFNgamma suppress the primary adaptive B cell response to plasmid encoded SU.

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Citations

Feb 12, 2002·Journal of Virology·Ingunn M StromnesKim J Hasenkrug
Jul 25, 2013·Viruses·Juliano Cezar Minardi da CruzYahia Chebloune
Aug 7, 2013·Viruses·Ramsés ReinaBeatriz Amorena
Aug 26, 2014·Viruses·Nancy StonosNiel Karrow
Aug 30, 2008·The Veterinary Journal·Ramsés ReinaBeatriz Amorena

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