Plasmid linkage of a bacteriocin-like substance in Streptococcus lactis subsp. diacetylactis strain WM4: transferability to Streptococcus lactis.

Applied and Environmental Microbiology
K M ScherwitzL L McKay

Abstract

Streptococcus lactis subsp. diacetylactis strain WM4 transferred lactose-fermenting and bacteriocin-producing (Bac+) abilities to S. lactis LM2301, a lactose-negative, streptomycin-resistant (Lac- Strr), plasmid-cured derivative of S. lactis C2. Three types of transconjugants were obtained: Lac+ Bac+, Lac+ Bac-, and Lac-Bac+.S. diacetylactis WM4 possessed plasmids of 88, 33, 30, 5.5, 4.8, and 3.8 megadaltons (Mdal). In Lac+ Bac+ transconjugants, lactose-fermenting ability was linked to the 33-Mdal plasmid and bacteriocin-producing ability to the 88-Mdal plasmid. Curing the 33-Mdal plasmid from Lac+ Bac+ transconjugants resulted in loss of lactose-fermenting ability but not bacteriocin-producing ability (Lac- Bac+). These strains retained the 88-Mdal plasmid. Curing of both plasmids resulted in a Lac- Bac- phenotype. The Lac+ Bac- transconjugant phenotype was associated with a recombinant plasmid of 55 or 65 Mdal. When these transconjugants were used as donors in subsequent matings, the frequency of Lac transfer was about 2.0 X 10(-2) per recipient plated, whereas when Lac+ Bac+ transconjugants served as donors, the frequency of Lac transfer was about 2.0 X 10(-5) per recipient plated. Also, Lac- Bac+ transconjugants were found ...Continue Reading

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Citations

Aug 1, 1993·Journal of Dairy Science·S F Barefoot, C G Nettles
Feb 11, 2015·Annual Review of Food Science and Technology·Larry McKay
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Sep 1, 1990·FEMS Microbiology Reviews·M J Gasson
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Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Oct 1, 1984·Applied and Environmental Microbiology·G P Davey
Aug 1, 1985·Applied and Environmental Microbiology·D C Graham, L L McKay
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Aug 1, 1990·Applied and Environmental Microbiology·C Ahn, M E Stiles
Apr 1, 1987·Applied and Environmental Microbiology·L R Steenson, T R Klaenhammer
May 1, 1989·Applied and Environmental Microbiology·M J van BelkumG Venema

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