PMID: 9171415Jun 1, 1997Paper

Plasmid maintenance functions of the large virulence plasmid of Shigella flexneri

Journal of Bacteriology
L RadnedgeStuart Austin

Abstract

The large virulence plasmid pMYSH6000 of Shigella flexneri contains a replicon and a plasmid maintenance stability determinant (Stb) on adjacent SalI fragments. The presence of a RepFIIA replicon on the SalI C fragment was confirmed, and the complete sequence of the adjacent SalI O fragment was determined. It shows homology to part of the transfer (tra) operon of the F plasmid. Stb stabilizes a partition-defective P1 miniplasmid in Escherichia coli. A 1.1-kb region containing a homolog of the F trbH gene was sufficient to confer stability. However, the trbH open reading frame could be interrupted without impairing stability. Deletion analysis implicated the involvement of two small open reading frames, STBORF1 and STBORF2, that fully overlap trbH in the opposite direction. These open reading frames are closely related to the vagC and vagD genes of the Salmonella dublin virulence plasmid and to open reading frame pairs in the F trbH region and in the chromosomes of Dichelobacter nodosus and Haemophilus influenzae. Stb appears to promote better-than-random distribution of plasmid copies and is a plasmid incompatibility determinant. The F homolog does not itself confer stability but exerts incompatibility against the activity of t...Continue Reading

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Citations

Sep 15, 2004·Molecular Genetics and Genomics : MGG·L G PuskásI Dusha
Mar 14, 2007·Microbial Ecology·Torben MartensThorsten Brinkhoff
Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kristoffer S Winther, Kenn Gerdes
Sep 26, 2006·FEMS Immunology and Medical Microbiology·Trevor K Wagner, Martha H Mulks
Sep 29, 2020·Frontiers in Microbiology·João Alves GamaFrancisco Dionisio
Oct 26, 2005·Applied Microbiology and Biotechnology·Michael BramucciVasantha Nagarajan
Apr 3, 2004·Journal of Bacteriology·Therese BrendlerStuart Austin
May 6, 2004·Journal of Bacteriology·Barbara FroehlichJune R Scott
Nov 26, 1998·Journal of Bacteriology·F Hayes
May 21, 2003·International Journal of Medical Microbiology : IJMM·M Isabel Fernandez, Philippe J Sansonetti

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