Plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli and Klebsiella pneumoniae isolated from market retail fruits in Guangzhou, China

Infection and Drug Resistance
F YangGuo-Bao Tian

Abstract

As a result of the growing prevalence of the plasmid-mediated mobile colistin resistance gene mcr-1 among Gram-negative bacteria, the surveillance of mcr-1 has been globally applied. In our study, we aimed to shed light on the possibility of transmission of mcr-1-resistant isolates through market retail fruits. Herein, 133 different fruit surface samples were collected and screened for the different MCR variants (mcr-1 to mcr-8) using PCR and confirmed with sequencing. We identify for the first time mcr-1-carrying Escherichia coli and Klebsiella pneumoniae from market retail fruits in Guangzhou, China. Minimum inhibitory concentrations were detected by the broth microdilution method. Liquid mating was performed to check the transferability of the mcr-1 gene. Pulsed field gel electrophoresis analysis of S1 nuclease-digested DNA and Southern blotting were performed to check the location of the mcr-1 gene. Then, whole-genome sequencing and in silico multilocus sequence typing analysis were performed. We showed that E. coli GB110 can mediate the spreading of antibiotic resistance genes through the food chain, while K. pneumoniae GB015 was considered to be the progenitor of the most successful multidrug-resistant clone. Since fruits...Continue Reading

Associated Datasets

Citations

Apr 14, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Claudia FloresCélia Maria C P A Romão
Apr 15, 2020·Emerging Microbes & Infections·Mohamed Abd El-Gawad El-Sayed AhmedGuo-Bao Tian
May 23, 2020·Scientific Reports·Jesus Rodriguez-ManzanoPantelis Georgiou
Feb 12, 2020·International Journal of Environmental Research and Public Health·Madubuike Umunna AnyanwuObichukwu Chisom Nwobi
Jul 30, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mehmet Cemal AdiguzelOrhan Sahin
May 8, 2021·Frontiers in Microbiology·Nicolle Lima BarbieriCatherine M Logue
Jul 3, 2021·Diseases·Ozioma Forstinus NwaborSarunyou Chusri

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Datasets Mentioned

BETA
PRJNA482733

Methods Mentioned

BETA
PCR
electrophoresis

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