PMID: 3322171May 1, 1986Paper

Plasmid-mediated contact haemolytic activity in Shigella species: correlation with penetration into HeLa cells

Annales De L'Institut Pasteur. Microbiology
P L ClercP J Sansonetti

Abstract

The main feature of virulent strains of Shigella is their ability to invade eucaryotic cells. This phenotype is both plasmid-mediated and temperature-regulated. In the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in S. flexneri, S. dysenteriae and S. sonnei. Detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees C. No soluble intra- or extracellular haemolytic activity could be detected. Dose-effect and electron microscopic studies demonstrated that direct contact of several virulent bacteria per erythrocyte was critical for haemolysis to occur. However, no local morphological alteration of the erythrocyte membrane at the site of contact with bacteria could be detected. Intensity of this haemolytic activity was fully correlated with the efficiency of penetration within HeLa cells, suggesting a common mechanism for induction of phagocytosis and lysis of erythrocytes.

References

Jun 1, 1979·Infection and Immunity·T L HaleP F Bonventre
May 1, 1978·The Journal of Infectious Diseases·S B Formal, R B Hornick
Jul 1, 1985·Infection and Immunity·A T MaurelliP J Sansonetti
Jan 1, 1985·The Journal of Infectious Diseases·S KabirQ Akhtar
Jan 1, 1973·Infection and Immunity·L E Ramm, H H Winkler
Oct 1, 1981·Infection and Immunity·P J SansonettiS B Formal
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Jan 1, 1984·Infection and Immunity·A T MaurelliR Curtiss
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Mar 1, 1959·The Journal of Experimental Medicine·Z A COHNT K SAWYER

❮ Previous
Next ❯

Citations

Mar 21, 2007·Molecular Microbiology·Andreas K J VeenendaalAriel J Blocker
Jan 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·William H ConradLalita Ramakrishnan
Mar 1, 1990·FEMS Microbiology Reviews·J L MartínezF Baquero
Jan 14, 1991·FEBS Letters·I E EhrmannE L Hewlett
Apr 12, 2001·Cellular Microbiology·R K ShawS Knutton
Jan 1, 1993·Infection and Immunity·S HåkanssonH Wolf-Watz
Sep 1, 1992·Infection and Immunity·C L PickettS S Jusuf
Apr 27, 2010·BMC Microbiology·Daniel SperandioAnnabelle Merieau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.