PMID: 7029254May 1, 1981Paper

Plasmid pKM101-dependent repair and mutagenesis in Escherichia coli cells with mutations lexB30 tif and zab-53 in the recA gene

Mutation Research
M Blanco, J E Rebollo

Abstract

Bacterial survival after UV irradiation was increased in E. coli K12 lexB30 and tif zab-53 mutants harboring plasmid pKM101. Mutagenesis in response to UV was observed in these bacteria which, in absence of pKM101, are not UV-mutable. The mutator effect observed in unirradiated wild-type cells containing pKM101 was higher than incubation at 30 degrees C with adenine than at 37 degrees C. This effect was still enhanced by tif mutation, even in the tif zab-53 strain, but it was abolished by lexB30 mutation. In the tif zab-53 (pKM101) strain, repair and mutagenesis of UV-irradiated phage lambda was observed, but not in the lexB30 mutant carrying pKM101. The pKM101 mutant, pGW1, was unable to protect tif zab-53 bacteria against killing by UV, whereas the protection of lexB30 was intermediate; moreover, it did not promote the mutator effect at 30 degrees C or enhance phage repair and mutagenesis in tif zab-53 cells. All UV-induced bacterial mutations in lexB30 (pKM101) strain were suppressors; in contrast, true revertants were found after UV irradiation of the tif zab-53 (pKM101) cells. We suggest that the constitutive activity of RecA protein is enough for the production of UV-promoted suppressor mutations, whereas true reversions ...Continue Reading

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·J E RebolloR Devoret
Mar 1, 1988·Molecular & General Genetics : MGG·J A VericatJ Barbé
Nov 1, 1986·Molecular & General Genetics : MGG·M BlancoV Aleixandre
Jan 1, 1995·Mutation Research·D G EnnisR Woodgate
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L PerryG C Walker
Jan 1, 1990·Environmental and Molecular Mutagenesis·N Abril, C Pueyo
May 1, 1995·Journal of Bacteriology·O I KulaevaR Woodgate
Sep 1, 1983·Journal of Bacteriology·S J Elledge, G C Walker
Mar 1, 1988·Journal of Bacteriology·J A VericatJ Barbé
Aug 1, 1982·Biochimie·M BlancoL M Botella

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