Plasmid Stability in Pseudomonas fluorescens in the Rhizosphere.

Applied and Environmental Microbiology
A J van der BijB J Lugtenberg

Abstract

Plasmids belonging to various incompatibility (Inc) groups were introduced into the efficiently root-colonizing strain Pseudomonas fluorescens WCS365, and their stabilities in complex and minimal media and in the rhizospheres of tomato, wheat, and potato plants grown under gnotobiotic conditions without selection pressure were tested. The IncP plasmid was found to be highly unstable under all conditions tested, whereas the IncQ and IncW plasmids showed intermediate stabilities and the plasmids pVSP41 and pWTT2081, for which the Inc group is unknown, both containing the origin of replication (rep) and stability (sta) regions of the Pseudomonas aeruginosa pVS1 replicon, were stably maintained under all conditions tested. Growth experiments in which cells of strain WCS365 carrying the plasmid pWTT2081 were grown in the presence of WCS365 without the plasmid showed that the presence of pWTT2081 acts as a burden. We conclude that pVSP41 and pWTT2081 are valuable as stable vectors for the functional analysis of genes involved in root colonization, provided that control cells carry the empty vector.

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Citations

Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Margarita M Camacho CarvajalGuido V Bloemberg
Jan 21, 2003·Journal of Bacteriology·Annabel H A ParretRené De Mot
Mar 7, 2003·Applied and Environmental Microbiology·Heike Schmidt-Eisenlohr, Christian Baron
Sep 24, 2005·Journal of Molecular Microbiology and Biotechnology·Catherine A Wright, Gwyn A Beattie
Jun 26, 2014·Journal of Applied Microbiology·J Y ZhouC C Dai
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·L C DekkersB J Lugtenberg
Nov 4, 2000·Molecular Plant-microbe Interactions : MPMI·W G MillerS E Lindow
Aug 30, 2002·Applied and Environmental Microbiology·Catherine A Axtell, Gwyn A Beattie
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Sandra de WeertBen J J Lugtenberg
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Thomas F C Chin-A-WoengGuido V Bloemberg
Jun 24, 2010·Applied Microbiology and Biotechnology·Aditi D BuchG Naresh Kumar
Jan 13, 1998·Molecular Plant-microbe Interactions : MPMI·L C DekkersB J Lugtenberg
Mar 31, 2006·Molecular Plant-microbe Interactions : MPMI·Seiko MakinoAdam J Bogdanove
Feb 17, 2018·Frontiers in Plant Science·Rosemarie WiltonKenneth M Kemner
Nov 26, 1997·Antimicrobial Agents and Chemotherapy·A A Salyers, C F Amábile-Cuevas
Nov 15, 1997·Applied and Environmental Microbiology·G V BloembergR Kolter

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