Plasmids for ectopic integration in Bacillus subtilis

Gene
A M Guérout-FleuryP Stragier

Abstract

Plasmids have been constructed that allow integration by a double recombination event at the thrC locus of the Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to the Escherichia coli lacZ gene. The plasmids contain an antibiotic (An) marker selectable in Bs, as well as an additional An marker outside of the region that can recombine into the chromosome. When used in conjunction with recipient strains containing a third An marker at their thrC locus, these plasmids allow easy identification of transformants issued from a marker exchange event without additional Campbell-type integration. The existing plasmids used for ectopic integration at the amyE locus have been modified similarly.

References

Jul 1, 1992·Biochimie·C Karmazyn-CampelliP Stragier
Apr 20, 1992·Journal of Molecular Biology·G Gonzy-TréboulP Stragier
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J YoungmanR Losick
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier

❮ Previous
Next ❯

Citations

Jul 19, 2005·Archives of Microbiology·Adam IwanickiMichal Obuchowski
May 24, 2003·Vaccine·Marco R OggioniGianni Pozzi
Sep 22, 1998·Nature Biotechnology·F ArigoniH Loferer
Mar 27, 2007·Nature Structural & Molecular Biology·Adam RothRonald R Breaker
Mar 12, 2002·Molecular Microbiology·P EichenbergerR Losick
Dec 12, 2001·Molecular Microbiology·S Little, A Driks
Jul 19, 2002·Molecular Microbiology·Michael A ZimmerGeorge W Ordal
Jan 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Susan H Fisher, Lewis V Wray
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ahmed GaballaJohn D Helmann
Dec 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel LópezRoberto Kolter
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·N Mani, B Dupuy
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jatin NarulaOleg A Igoshin
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pascale B BeauregardRoberto Kolter
Nov 12, 2002·The EMBO Journal·Marc D Sharp, Kit Pogliano
Jan 6, 2004·Nucleic Acids Research·Ali NahviRonald R Breaker
Jul 10, 2007·Nucleic Acids Research·Irina Saraiva FrancoIsabel de Sá-Nogueira
Dec 28, 2002·Genes & Development·Angelica Abanes-De MelloKit Pogliano
Apr 3, 2008·Genes & Development·Hera VlamakisRoberto Kolter
Jul 17, 2009·Genes & Development·Daniel LópezRoberto Kolter
Mar 29, 2011·Applied and Environmental Microbiology·Xiaoman XuPing Xu
Jun 12, 2012·Applied and Environmental Microbiology·Tamara HoffmannErhard Bremer
Jul 25, 2006·Infection and Immunity·Tracey L CampbellEric D Brown
Mar 5, 2013·Journal of Bacteriology·Amy T Cavanagh, Karen M Wassarman
Apr 17, 2007·Journal of Bacteriology·Adrian J JervisAnne Moir

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.