Plasmin activity is required for myogenesis in vitro and skeletal muscle regeneration in vivo.

Blood
Mònica SuelvesPura Muñoz-Cánoves

Abstract

Plasmin, the primary fibrinolytic enzyme, has a broad substrate spectrum and is implicated in biologic processes dependent upon proteolytic activity, such as tissue remodeling and cell migration. Active plasmin is generated from proteolytic cleavage of the zymogen plasminogen (Plg) by urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA). Here, we have investigated the role of plasmin in C2C12 myoblast fusion and differentiation in vitro, as well as in skeletal muscle regeneration in vivo, in wild-type and Plg-deficient mice. Wild-type mice completely repaired experimentally damaged skeletal muscle. In contrast, Plg(-/-) mice presented a severe regeneration defect with decreased recruitment of blood-derived monocytes and lymphocytes to the site of injury and persistent myotube degeneration. In addition, Plg-deficient mice accumulated fibrin in the degenerating muscle fibers; however, fibrinogen depletion of Plg-deficient mice resulted in a correction of the muscular regeneration defect. Because we found that uPA, but not tPA, was induced in skeletal muscle regeneration, and persistent fibrin deposition was also reproducible in uPA-deficient mice following injury, we propose that fibrinolysis by ...Continue Reading

References

Jan 1, 1990·Acta Neuropathologica·H KawaiM Iwasa
Nov 15, 1985·Science·H M BlauC Webster
Jan 1, 1983·Cell·C B Couch, W J Strittmatter
Oct 19, 1995·Nature·T Yagami-HiromasaA Fujisawa-Sehara
Jan 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C W Guérin, P C Holland
Aug 1, 1993·Experimental Cell Research·T A RobertsonJ M Papadimitriou
Mar 1, 1996·Nature Medicine·J RomerK Dano
May 15, 1996·Genes & Development·L A MegeneyM A Rudnicki
Feb 1, 1997·Muscle & Nerve·A Pimorady-EsfahaniP G McMenamin
Mar 3, 1997·The Journal of Experimental Medicine·A R KitchingP G Tipping
May 30, 1998·The Journal of Biological Chemistry·E GredingerE Bengal
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·J P IrigoyenY Nagamine

❮ Previous
Next ❯

Citations

Mar 23, 2013·Stem Cells and Development·Alexander BirbrairOsvaldo Delbono
Feb 5, 2009·Tissue Engineering. Part C, Methods·Alastair Khodabukus, Keith Baar
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Angels Díaz-RamosRoser López-Alemany
Jul 30, 2011·Skeletal Muscle·Christopher J MannPura Muñoz-Cánoves
Jan 15, 2009·Folia Histochemica Et Cytobiologica·Ewa SewerynJadwiga Pietkiewicz
Mar 20, 2013·Mediators of Inflammation·Yacine KharrazPura Muñoz-Cánoves
Jan 21, 2014·Annual Review of Food Science and Technology·Peter P Purslow
Jun 6, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Asami YoshidaKenji Hara
Mar 8, 2014·European Journal of Applied Physiology·Ryan M FrancisJeffrey T Lemmer
Oct 23, 2008·The Journal of Biological Chemistry·Jianming LiuStephen J Kaufman
Sep 6, 2007·The Journal of Cell Biology·Mònica SuelvesPura Muñoz-Cánoves
Jan 4, 2012·The Journal of Cell Biology·Esther ArditePura Muñoz-Cánoves
Feb 2, 2011·Expert Opinion on Biological Therapy·Isabel AndiaNicola Maffulli
Jun 24, 2010·Experimental Cell Research·Antonio L Serrano, Pura Muñoz-Cánoves
Apr 4, 2007·Journal of Thrombosis and Haemostasis : JTH·O MatsuoS S Smyth
Jan 13, 2012·Chembiochem : a European Journal of Chemical Biology·Joakim E Swedberg, Jonathan M Harris
Mar 29, 2008·Cell Stem Cell·Beate HeissigKoichi Hattori
Oct 27, 2004·Developmental Biology·Patrick SealeMichael A Rudnicki
Oct 7, 2016·Micron : the International Research and Review Journal for Microscopy·Mohamed A A MahdyYoshinao Z Hosaka
Aug 18, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nicholas A MignemiJonathan G Schoenecker
Feb 7, 2017·Nature Reviews. Immunology·James G Tidball
Feb 25, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wen-Chung TsaiJong-Hwei S Pang
May 18, 2018·The American Journal of Sports Medicine·Wen-Chung TsaiJong-Hwei S Pang
Aug 31, 2018·International Immunology·Yumiko Oishi, Ichiro Manabe
Jan 24, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Rosemarijn RenckensTom van der Poll
Feb 18, 2005·American Journal of Physiology. Cell Physiology·Timothy J KohThomas H Sisson
Jan 13, 2004·Physiological Reviews·Sophie B P Chargé, Michael A Rudnicki
Apr 8, 2020·American Journal of Physiology. Endocrinology and Metabolism·Lian LiuJonathan M Peake
Jun 7, 2003·American Journal of Physiology. Endocrinology and Metabolism·Stéphanie DuguezDamien Freyssenet
Mar 23, 2017·Journal of Bone and Mineral Metabolism·Yukinori TamuraHiroshi Kaji
Sep 20, 2018·Journal of Experimental Orthopaedics·Stephanie N Moore-LotridgeJonathan G Schoenecker
Oct 1, 2020·International Journal of Molecular Sciences·Fasih Ahmad Rahman, Matthew Paul Krause
Jun 18, 2020·Research and Practice in Thrombosis and Haemostasis·Breanne H Y GibsonJonathan G Schoenecker
May 14, 2018·Cell and Tissue Research·Mohamed A A Mahdy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.