Plasmin produces an E-cadherin fragment that stimulates cancer cell invasion

Biological Chemistry
Filip RyniersErik Bruyneel

Abstract

Matrix metalloproteases from the cell surface cleave an 80 kDa E-cadherin fragment (sE-CAD) that induces invasion of cancer cells into collagen type I and inhibits cellular aggregation. Conditioned media from MDCKts.srcCl2 cells at 40 degrees C and 35 degrees C, PCm.src5 and COLO-16 cells at 37 degrees C contained spontaneously released sE-CAD; these 48 h old conditioned media were capable of inhibiting E-cadherin functions in a paracrine way. Here we show direct cleavage of the extracellular domain of E-cadherin by the serine protease plasmin. sE-CAD released by plasmin inhibits E-cadherin functions as evidenced by induction of invasion into collagen type I and inhibition of cellular aggregation. This functional inhibition by sE-CAD was reversed by aprotinin or by immunoadsorption on protein Sepharose 4 fast flow beads with antibodies against the extracellular part of E-cadherin. Our results demonstrate that plasmin produces extracellular E-cadherin fragments which regulate E-cadherin function in cells containing an intact E-cadherin/catenin complex.

References

Jul 1, 1987·Journal of Cellular Biochemistry·M J WheelockC H Damsky
Feb 1, 1995·Journal of Clinical Pathology·R E BanksP J Selby
Mar 1, 1994·British Journal of Cancer·M KatayamaI Kato
Feb 1, 1994·Current Opinion in Genetics & Development·G A Rodrigues, M Park
Jan 1, 1996·Enzyme & Protein·P Mignatti, D B Rifkin
Dec 11, 1995·International Journal of Cancer. Journal International Du Cancer·S A RabbaniA P Mazar
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·P A AndreasenM J Duffy
Nov 20, 1997·Journal of Cellular Physiology·M MareelM Bracke
Oct 29, 1998·British Journal of Cancer·J GofukuM Monden
Nov 15, 2000·The Journal of Biological Chemistry·U SteinhusenO Huber
Apr 4, 2002·Biological Chemistry·Olivier De Wever, Marc Mareel

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Citations

Oct 24, 2007·Clinical & Experimental Metastasis·Olivier De WeverMarc Bracke
Mar 20, 2003·Mutation Research·Sheau-Fung ThaiJames C Fuscoe
Jan 27, 2005·Thyroid : Official Journal of the American Thyroid Association·Ana Sofia RochaPaula Soares
Feb 9, 2010·Cardiovascular Research·Helen WilliamsSarah J George
Feb 17, 2006·Genes & Development·Vasilena GochevaJohanna A Joyce
Nov 19, 2010·Cancer Research·Sonya E L Craig, Susann M Brady-Kalnay
Apr 4, 2002·Biological Chemistry·Olivier De Wever, Marc Mareel
May 2, 2012·ISRN Oncology·Suttichai Krisanaprakornkit, Anak Iamaroon
Jun 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Thorsten MaretzkyPaul Saftig
Oct 10, 2006·Clinical & Experimental Metastasis·L DeryckeE Van Aken
Jul 16, 2009·Cellular and Molecular Life Sciences : CMLS·Angela DittmerJürgen Dittmer
Mar 24, 2012·Biochimica Et Biophysica Acta·Fausto J RodriguezPanos Z Anastasiadis
Jul 8, 2003·The Journal of Pathology·Olivier De Wever, Marc Mareel
May 6, 2008·Biochimica Et Biophysica Acta·Matteo MozzicafreddoMauro Angeletti
Dec 4, 2009·Journal of Cellular Physiology·Lara LapyckyjMónica Hebe Vazquez-Levin
Dec 26, 2015·International Journal of Cancer. Journal International Du Cancer·Qi-Ping HuShi-Cang Yu
May 23, 2012·Biochimica Et Biophysica Acta·Joana ParedesRaquel Seruca
Feb 9, 2012·Dermatology Research and Practice·Miquella G ChavezLaurie G Hudson
Oct 12, 2010·The Journal of International Medical Research·R-Y ZhangY-Q Tang
Oct 2, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Michelle L GervaisMichael Ohh
May 31, 2003·Circulation Research·Elizabeth B UglowSarah J George
Sep 26, 2006·International Journal of Cancer. Journal International Du Cancer·Lara DeryckeMarc Bracke
Feb 3, 2007·Journal of the American Society of Nephrology : JASN·Guoqiang ZhangAllison A Eddy
Aug 19, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kirsty A Green, Leif R Lund
May 16, 2015·Arthritis Research & Therapy·Erika H NossMichael B Brenner
May 11, 2006·Skin Pharmacology and Physiology·K BillionC M Niessen
May 10, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Olivier MeilhacEduardo Anglés-Cano
Jul 26, 2002·The Journal of Biological Chemistry·Hidayatullah G MunshiM Sharon Stack
Jul 5, 2016·Oncotarget·Yusi LiuLingyun Zhou
Mar 29, 2003·Physiological Reviews·Marc Mareel, Ancy Leroy
Mar 6, 2003·Journal of Cancer Research and Clinical Oncology·Yingming JiangXuetao Cao
Apr 26, 2020·Cells·Michele Sommariva, Nicoletta Gagliano

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