Plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism and endometriosis. Influence of PAI-1 polymorphism on PAI-1 antigen and mRNA expression

Thrombosis Research
Luis Andres RamonAmparo Estelles

Abstract

Endometriosis is a benign gynecologic disease with a high prevalence. It is a multifactorial and polygenic entity in which the fibrinolytic system may be implicated. The objective of this study was to evaluate the plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism in a group of women with and without endometriosis and to analyze the influence of this polymorphism in PAI-1 expression in endometrial tissue and peritoneal fluid. In 389 women (170 patients with endometriosis and 219 controls) PAI-1 4G/5G polymorphism was determined by PCR amplification using allele-specific primers. Quantitative real-time RT-PCR assay was used to quantify PAI-1 mRNA and PAI-1 antigen (ag) levels were quantified by ELISA. The genotype and allele frequencies of PAI-1 4G/5G polymorphism did not differ significantly between patients and controls. Control women with the 4G/4G genotype had higher endometrial PAI-1ag (P=0.026) and mRNA (P=0.014) levels than those with the 5G/5G genotype. Control carrying the 4G/4G genotype tended to have higher peritoneal fluid PAI-1ag levels than those carrying the 5G/5G genotype. Moreover, PAI-1ag levels in peritoneal fluid were higher in patients than in controls (P=0.003). The PAI-1 genotype distribution was...Continue Reading

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Citations

Aug 9, 2011·Genetic Testing and Molecular Biomarkers·Ariel BrandesCaio P Barbosa
Jun 16, 2011·Human Reproduction Update·K E MayC M Becker
Jul 18, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Davide GentiliniAnna Maria di Blasio
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Oct 22, 2009·Reproductive Sciences·Olga KlinkovaKathleen M Eyster
Aug 2, 2017·International Journal of Molecular Sciences·Yao YeViktoria von Schönfeldt
Mar 9, 2018·Biology of Reproduction·Anna BuiguesRaúl Gómez
Apr 21, 2016·The Cochrane Database of Systematic Reviews·Devashana GuptaVicki Nisenblat
Dec 11, 2019·Human Reproduction Update·Loren MéarFrançois Vialard
Oct 23, 2020·Reproductive Sciences·Mili ThakurAwoniyi O Awonuga

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