Plasminogen activator inhibitor type 2: a regulator of monocyte proliferation and differentiation.

Blood
Hong YuR L Medcalf

Abstract

We have explored the role of plasminogen activator inhibitor type 2 (PAI-2) in THP-1 monocyte-like cells. These cells possess a mutation in the PAI-2 gene and do not produce an active PAI-2 protein. Transfection of THP-1 cells with plasmids expressing active PAI-2 reduced the cells' inherent adhesive properties and decreased the rate of cell proliferation. THP-1 cells expressing active PAI-2 also displayed an altered phenotype in response to phorbol ester-induced differentiation that was concomitant with a reduction in CD14 expression. THP-1 cells transfected with a variant PAI-2 containing a mutation in the reactive center (PAI-2(Ala380)) displayed no noticeable change in any of these parameters, suggesting the involvement of a PAI-2-sensitive serine protease(s). The antiproliferative effect of PAI-2 was attenuated by treating the PAI-2-expressing THP-1 cells with recombinant urokinase (u-PA), suggesting that PAI-2 was disruptive of a u-PA/u-PA receptor signaling pathway initiated on the cell surface. Consistent with this, treatment of wild-type THP-1 cells with recombinant PAI-2 also caused a reduction in cellular proliferation. These results implicate endogenous PAI-2 as a modulator of monocyte adhesion, proliferation, and d...Continue Reading

References

Jul 1, 1978·The Journal of Experimental Medicine·A Granelli-Piperno, E Reich
Mar 11, 1991·The Journal of Clinical Investigation·A R Nusrat, H A Chapman
Apr 15, 1989·European Journal of Biochemistry·J C KirchheimerB R Binder
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·I M Nilsson
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·B M MuellerW E Laug
Apr 26, 1996·The Journal of Biological Chemistry·P Mikus, T Ny
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·P A AndreasenM J Duffy
Feb 7, 1998·The Journal of Biological Chemistry·I DumlerD C Gulba
Jan 14, 1999·The Journal of Investigative Dermatology·T HibinoP F Goetinck
Feb 16, 1999·The Journal of Investigative Dermatology·P J Jensen, R M Lavker
Mar 11, 1999·The Journal of Laboratory and Clinical Medicine·M R GyetkoG Toews
Aug 10, 1999·Journal of Virology·D R ShafrenA Suhrbier

❮ Previous
Next ❯

Citations

Mar 25, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Andrea SadlonovaAndra R Frost
Mar 12, 2004·Thrombosis Research·Iwona KucharewiczAnna Bodzenta-Łukaszyk
Jan 30, 2010·Leukemia·E VoissetP De Sepulveda
Jun 13, 2008·Nature Reviews. Cancer·David R CroucherMarie Ranson
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shih-Yi ChuangLi-Chung Hsu
Jul 5, 2013·PloS One·Carolyn GlassArchibald Perkins
Sep 24, 2005·The FEBS Journal·Robert L Medcalf, Stan J Stasinopoulos
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·R L Medcalf
Feb 19, 2008·Clinical and Experimental Immunology·A A HigaziR Safadi
May 24, 2008·Tissue Antigens·M S J ManganP I Bird
Jan 13, 2012·Chembiochem : a European Journal of Chemical Biology·Joakim E Swedberg, Jonathan M Harris
Aug 22, 2014·Journal of Thrombosis and Haemostasis : JTH·E E Gardiner, R L Medcalf
Mar 20, 2014·Cancer Medicine·Wayne A SchroderAndreas Suhrbier
Mar 12, 2013·Experimental Cell Research·Joanna BoncelaCzeslaw S Cierniewski
Dec 6, 2011·Atherosclerosis·Bianca Fuhrman
Mar 21, 2017·The Journal of Allergy and Clinical Immunology·Raphaël MarluLaurence Bouillet
Feb 13, 2003·The Journal of Biological Chemistry·Hong YuRobert L Medcalf
Jul 22, 2008·Journal of Leukocyte Biology·Michael Bots, Jan Paul Medema
Apr 26, 2006·The Journal of Biological Chemistry·Séverine CathelinEric Solary
Aug 23, 2006·The Journal of Biological Chemistry·Grant A DarnellAndreas Suhrbier
Feb 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wayne A SchroderAndreas Suhrbier
Jun 18, 2020·International Journal of Molecular Sciences·Chuanyin LiRonggui Hu
Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshitomo HamanoKazuo Suzuki
Jun 8, 2021·HemaSphere·Robert L Medcalf, Charithani B Keragala
Jun 10, 2010·Journal of Proteome Research·Nilubon SinghtoVisith Thongboonkerd

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.