Plasminogen activator inhibitor type 2 is expressed in keratinocytes during re-epithelialization of epidermal defects

The British Journal of Dermatology
M J BechtelB M Schaefer

Abstract

Plasminogen activation is observed in the human epidermis during re-epithelialization of epidermal defects. The activation reaction depends on plasminogen activators (PAs) associated with re-epithelializing keratinocytes. PA inhibitor type 2 (PAI-2) is thought to be a major epidermal PA inhibitor in keratinocytes. However, no data are available on the expression of PAI-2 in keratinocytes during epidermal regeneration. We have therefore analysed PAI-2 at the mRNA and protein level in keratinocyte cultures as well as in epidermal lesions in which re-epithelializing keratinocytes were apparent. We found that PAI-2 expression at the mRNA and protein level was negatively correlated with the cell density in regular keratinocyte cultures. In organotypic cocultures, in which the transition from a re-epithelializing to a sedentary phenotype can be studied, PAI-2 was most strongly expressed in early cultures prior to formation of a differentiated epidermis-like structure. We found a strong expression of PAI-2 in keratinocytes that re-epithelialized dermal burn wounds or lesions caused by the autoimmune blistering disease pemphigus vulgaris. Our results suggest that not only PAs, but also a major PA inhibitor, PAI-2, are expressed in kera...Continue Reading

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Citations

Mar 22, 2006·Cell and Tissue Research·Miriam Hernández-QuinteroFederico Castro-Muñozledo
Apr 30, 2002·Cell Biochemistry and Function·Ronan O'LearyEdward J Wood
Nov 25, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Abdoelwaheb El GhalbzouriMaria Ponec
Sep 29, 2004·Colloids and Surfaces. B, Biointerfaces·Jun YanJin Yang

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