PMID: 2497739Apr 15, 1989Paper

Plasminogen activator inhibitor-type I is a major biosynthetic product of retinal microvascular endothelial cells and pericytes in culture

The Biochemical Journal
A E CanfieldM E Grant

Abstract

Previous studies have shown that a glycoprotein of Mr 47,000 (designated Gp47) is a major biosynthetic product of retinal endothelial cells in vitro (Canfield, Schor, West, Schor & Grant (1987) Biochem. J. 246, 121-129). We now present data indicating that (a) an identical protein is secreted by bovine retinal pericytes, (b) this protein is plasminogen activator inhibitor-type I (PAI-1), as revealed by immunoprecipitation with specific antibodies and reverse fibrin zymography, and (c) retinal endothelial cells and pericytes synthesize different species of matrix macromolecules, that is: type IV collagen is the major collagen secreted by endothelial cells, whereas pericytes produce predominantly type I collagen; fibronectin and thrombospondin are synthesized by both cell types. Our studies also indicate that PAI-1 is produced, albeit at considerably lower levels, by large vessel vascular cells (aortic endothelial and smooth muscle cells) and human skin fibroblasts. PAI-1 produced by human skin fibroblasts appears to be a distinct molecular species compared to its bovine counterpart as assessed by its slower mobility on SDS/polyacrylamide-gel electrophoresis. The potential significance of elevated PAI-1 production by retinal endo...Continue Reading

Citations

Jul 6, 2005·Progress in Retinal and Eye Research·Paul HiscottDavid J Armstrong
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·M Paulsson
Feb 26, 2011·Fluids and Barriers of the CNS·Drew BonkowskiPaula Dore-Duffy
Apr 21, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Patricia A Soucy, Lewis H Romer
Nov 1, 1991·Diabetic Medicine : a Journal of the British Diabetic Association·J SkrhaP Stolba
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Toshiyuki KajiThomas N Wight
Apr 1, 1994·Journal of Cellular Physiology·A E Canfield, A M Schor
Aug 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A M SchorS L Schor
Sep 1, 1997·The Journal of Clinical Endocrinology and Metabolism·T Merimee

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