Plasmodium falciparum merozoite surface protein 1 (MSP-1)-MSP-3 chimeric protein: immunogenicity determined with human-compatible adjuvants and induction of protective immune response.

Infection and Immunity
Suman MazumdarVirander Singh Chauhan

Abstract

A chimeric gene, MSP-Fu(24), was constructed by genetically coupling immunodominant, conserved regions of the two leading malaria vaccine candidates, Plasmodium falciparum merozoite surface protein 1 (C-terminal 19-kDa region [PfMSP-1(19)]) and merozoite surface protein 3 (11-kDa conserved region [PfMSP-3(11)]). The recombinant MSP-Fu(24) protein was produced in Escherichia coli cells and purified to homogeneity by a two-step purification process with a yield of approximately 30 mg/liter. Analyses of conformational properties of MSP-Fu(24) using PfMSP-1(19)-specific monoclonal antibody showed that the conformational epitopes of PfMSP-1(19) that may be critical for the generation of the antiparasitic immune response remained intact in the fusion protein. Recombinant MSP-Fu(24) was highly immunogenic in mice and in rabbits when formulated with two different human-compatible adjuvants and induced an immune response against both PfMSP-1(19) and PfMSP-3(11). Purified anti-MSP-Fu(24) antibodies showed invasion inhibition of P. falciparum 3D7 and FCR parasites, and this effect was found to be dependent on antibodies specific for the PfMSP-1(19) component. The protective potential of MSP-Fu(24) was demonstrated by in vitro parasite gro...Continue Reading

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Citations

Aug 26, 2014·Jundishapur Journal of Microbiology·Adel EbrahimzadehAli Jamshidi
Oct 16, 2015·Vaccine·Simon J DraperSumi Biswas
Jan 28, 2017·Immunotherapy·Manuel E PatarroyoJorge Aza-Conde
May 3, 2014·Clinical and Vaccine Immunology : CVI·Puneet K GuptaVirander S Chauhan
Mar 30, 2017·Proteome Science·Samuel Antwi-BaffourBen Gyan

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