PMID: 8943149Nov 12, 1996Paper

Plasmodium falciparum protein kinase 5 and the malarial nuclear division cycles

Molecular and Biochemical Parasitology
R GraeserBarbara Kappes

Abstract

In the course of our studies on cell cycle regulation mechanisms of Plasmodium falciparum, we investigated expression pattern, kinase activity, and localization of PfPK5, a putative malarial member of the family of cyclin-dependent protein kinase (cdks). The kinase was immunoprecipitated from parasites of selected stages and from parasites blocked with the cell-cycle inhibitor aphidicolin. An elevated kinase activity of PfPK5 from aphidicolin-blocked cells suggested that the enzyme might be implicated in the regulation of the parasite's S-phase. To further investigate this hypothetical function, parasite cultures were treated with the specific cdk inhibitors flavopiridol and olomoucine, which act on PfPK5 in vitro at similar concentrations as on other cdks. When applied during the nuclear division cycles of the parasite, both drugs markedly inhibited the DNA synthesis, as predicted from our proposition that PfPK5 is necessary to activate or maintain the parasite S-phase. Immunolocalization studies provide further evidence for this potential role of PfPK5.

References

Apr 1, 1992·Current Opinion in Cell Biology·A B Pardee, K Keyomarsi
Aug 1, 1992·Molecular and Biochemical Parasitology·K A CreedonT E Wellems
Jan 15, 1992·Biochemical and Biophysical Research Communications·R V GuntakaV D Reddy
Jan 1, 1985·Transactions of the Royal Society of Tropical Medicine and Hygiene·M O de Rojas, M Wasserman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz
Feb 1, 1983·Experimental Parasitology·J D ChulayC L Diggs
Jan 1, 1984·Molecular and Biochemical Parasitology·J Inselburg, H S Banyal
Jan 1, 1995·Biology of the Cell·R T AbrahamD Fagot
Jun 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E A Nigg
Mar 1, 1995·Molecular and Biochemical Parasitology·C DoerigR Carter
Mar 1, 1995·Molecular and Biochemical Parasitology·B J BennettR L Coppel
Jan 1, 1995·Molecular and Biochemical Parasitology·H J Longhurst, A A Holder
Sep 1, 1994·European Journal of Biochemistry·J VeselýS Leclerc
Jun 1, 1993·Trends in Biochemical Sciences·J Pines
Jul 1, 1996·Molecular and Biochemical Parasitology·R GraeserB Kappes

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Citations

Nov 25, 2003·Journal of Molecular Graphics & Modelling·Susan M Keenan, William J Welsh
Nov 19, 2002·International Journal for Parasitology·Christian DoerigDebopam Chakrabarti
Nov 18, 1997·International Journal for Parasitology·W Trager, J B Jensen
Apr 11, 2001·Molecular and Biochemical Parasitology·P HassanJ C Mottram
May 30, 2002·Molecular and Biochemical Parasitology·María I SantoriMaría T Téllez-Iñón
Nov 6, 1998·Molecular and Biochemical Parasitology·R VinkenoogA P Waters
Nov 3, 2004·Genome Research·Karine G Le RochElizabeth A Winzeler
Dec 5, 2012·Antimicrobial Agents and Chemotherapy·Elaine B BohórquezSteven R Meshnick
Aug 9, 2007·Future Microbiology·Barbara Kappes, Petra Rohrbach
Oct 8, 1999·Parasitology Today·B KappesR Graeser
Sep 25, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sandrine HouzéLuc Demange
Oct 11, 2008·Trends in Parasitology·Christian DoerigNorman C Waters
Apr 28, 2009·Molecular and Biochemical Parasitology·Fernanda C KoyamaCélia R S Garcia
Jan 3, 2012·Parasitology International·Kentaro KatoTatsuya Iwanaga
Jan 31, 2003·Expert Opinion on Therapeutic Targets·Norman C Waters, Jeanne A Geyer
Feb 19, 2015·The Journal of Antibiotics·Aki IshiyamaKazuhiko Otoguro
Mar 25, 2010·Molecular and Biochemical Parasitology·Dayadevi JirageNorman C Waters
Aug 12, 2009·Molecular and Biochemical Parasitology·Duk-Won Doug ChungKarine G Le Roch
Mar 29, 2018·Parasites & Vectors·Holly MatthewsCatherine J Merrick
Feb 28, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Brian T GrimbergPeter A Zimmerman
Dec 13, 2005·The Journal of Biological Chemistry·Martin GengenbacherBarbara Kappes
Sep 1, 2009·Expert Review of Clinical Pharmacology·Athar AlamUday Bandyopadhyay
Sep 27, 2019·Future Medicinal Chemistry·Marilia Nn LimaCarolina Horta Andrade

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