Plasmolysis and cell shape depend on solute outer-membrane permeability during hyperosmotic shock in E. coli

Biophysical Journal
Teuta Pilizota, Joshua W Shaevitz

Abstract

The concentration of chemicals inside the bacterial cytoplasm generates an osmotic pressure, termed turgor, which inflates the cell and is necessary for cell growth and survival. In Escherichia coli, a sudden increase in external concentration causes a pressure drop across the cell envelope that drives changes in cell shape, such as plasmolysis, where the inner and outer membranes separate. Here, we use fluorescence imaging of single cells during hyperosmotic shock with a time resolution on the order of seconds to examine the response of cells to a range of different conditions. We show that shock using an outer-membrane impermeable solute results in total cell volume reduction with no plasmolysis, whereas a shock caused by outer-membrane permeable ions causes plasmolysis immediately upon shock. Slowly permeable solutes, such as sucrose, which cross the membrane in minutes, cause plasmolysis to occur gradually as the chemical potential equilibrates. In addition, we quantify the detailed morphological changes to cell shape during osmotic shock. Nonplasmolyzed cells shrink in length with an additional lateral size reduction as the magnitude of the shock increases. Quickly plasmolyzing cells shrink largely at the poles, whereas gr...Continue Reading

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Citations

Nov 22, 2013·Journal of the Royal Society, Interface·Marcel ReuterIan R Booth
Aug 28, 2015·Colloids and Surfaces. B, Biointerfaces·Jana Schwarz-LinekWilson C K Poon
Nov 25, 2014·Biophysical Journal·Teuta Pilizota, Joshua W Shaevitz
Apr 15, 2015·The Journal of General Physiology·Janet M Wood
Jul 6, 2016·International Journal of Food Microbiology·Aline MétrisDelphine Ropers
Sep 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Renata BudaTeuta Pilizota
Dec 14, 2016·Scientific Reports·Keiran StevensonTeuta Pilizota
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enrique RojasKerwyn Casey Huang
Aug 19, 2015·Infection and Immunity·Azad EshghiMathieu Picardeau
Oct 13, 2018·Biotechnology and Applied Biochemistry·Wenjian MaXiaoyuan Wang
Jan 1, 2016·Nanomaterials·Monika MortimerPatricia A Holden
Feb 20, 2019·Physical Review. E·Md Zulfikar AliRanjan Mukhopadhyay
Feb 15, 2020·Physical Review Letters·Alexandru ParaschivAnđela Šarić
Apr 15, 2020·Nature Communications·Marion Mathelié-GuinletYves F Dufrêne
Dec 21, 2019·Scientific Reports·Yao-Kuan WangChien-Jung Lo
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Tathiane Ferroni PassosClovis Wesley Oliveira de Souza
Sep 10, 2018·New Biotechnology·Amber L DoreyCatarina Marinho

Related Concepts

Plasma Membrane
Cell Membrane Permeability
Alkalescens-Dispar Group
Ions
Osmosis
Osmotic Stress
Cell Growth
Cytoplasm
Escherichia coli
Ions

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