Plasmonic Cu2- x S y Se1- y Nanoparticles Catalyzed Click Chemistry Reaction for SERS Immunoassay of Cancer Biomarker

Analytical Chemistry
Lin YangCheng Zhi Huang

Abstract

Nature enzyme-based immunoassays have been widely used in fundamental scientific research and clinical diagnosis. However, the limitations of natural enzyme, such as the low physical/chemical stability or susceptibility to protein denaturation, greatly restrained its applications. In this article, we reported a new enzyme-free SERS immunoassay by utilizing plasmonic Cu2- xS ySe1- y nanoparticles (NPs) as nanocatalyst to catalyze the click chemistry between the azido and alkynyl substrate which is used as the SERS signal reporter. The unique vibration of C≡C of alkynyl in the Raman-silent region (1800-2800 cm-1) is not overlapped with the signals of the other conventional Raman reporters or endogenous biological species, and thus it can make sure the enzyme-free SERS immunoassay has high selectivity and sensitivity. As a proof of concept, prostate-specific antigen (PSA), a biomarker of prostate cancer in blood, has been detected. The SERS immunoassay shows good analytical performance for PSA in the range of 3-120 ng mL-1, and it has been successfully applied to detect PSA in the serum samples of prostate cancer patients, proving that the proposed enzyme-free SERS immunoassay has great potential in the clinical diagnosis of cancer.

References

Jul 26, 2008·Nature Nanotechnology·Lizeng GaoXiyun Yan
Jan 9, 2009·Angewandte Chemie·Atul AsatiJ Manuel Perez
Jun 15, 2010·Journal of the American Chemical Society·Sasanka DekaLiberato Manna
Mar 10, 2011·Angewandte Chemie·Weisi QuXingyu Jiang
Oct 7, 2011·Chemical Communications : Chem Comm·Yi Juan LongCheng Zhi Huang
Feb 19, 2013·Trends in Biotechnology·Marc VendrellYoung-Tae Chang
Oct 17, 2013·Journal of the American Chemical Society·Yi XieLiberato Manna
Dec 3, 2014·Current Opinion in Chemical Biology·Senlian HongXing Chen
Feb 18, 2017·Analytical Chemistry·Yongmei YinDingbin Liu
Mar 1, 2017·Physical Chemistry Chemical Physics : PCCP·Hong Yan ZouCheng Zhi Huang
Jul 21, 2017·Journal of Agricultural and Food Chemistry·Meng-Lei XuBing Zhao
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal

❮ Previous
Next ❯

Citations

Aug 21, 2020·ACS Applied Materials & Interfaces·Juan WangLoes I Segerink

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.