Plastic compression of a collagen gel forms a much improved scaffold for ocular surface tissue engineering over conventional collagen gels

Journal of Biomedical Materials Research. Part a
Shengli MiChe John Connon

Abstract

We compare the use of plastically compressed collagen gels to conventional collagen gels as scaffolds onto which corneal limbal epithelial cells (LECs) are seeded to construct an artificial corneal epithelium. LECs were isolated from bovine corneas (limbus) and seeded onto either conventional uncompressed or novel compressed collagen gels and grown in culture. Scanning electron microscopy (SEM) results showed that fibers within the uncompressed gel were loose and irregularly ordered, whereas the fibers within the compressed gel were densely packed and more evenly arranged. Quantitative analysis of LECs expansion across the surface of the two gels showed similar growth rates (p > 0.05). Under SEM, the LECs, expanded on uncompressed gels, showed a rough and heterogeneous morphology, whereas on the compressed gel, the cells displayed a smooth and homogeneous morphology. Transmission electron microscopy (TEM) results showed the compressed scaffold to contain collagen fibers of regular diameter and similar orientation resembling collagen fibers within the normal cornea. TEM and light microscopy also showed that cell-cell and cell-matrix attachment, stratification, and cell density were superior in LECs expanded upon compressed colla...Continue Reading

References

Apr 23, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hani A AwadDavid L Butler
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Wafa M ElbjeiramiJennifer L West
Nov 25, 2003·Tissue Engineering·Joanne GarvinAlbert J Banes
Jun 8, 2004·Journal of Biomechanical Engineering·Preethi L Chandran, Victor H Barocas
Jun 29, 2004·Progress in Retinal and Eye Research·Tapio IhanamäkiEero Vuorio
Dec 14, 2004·Tissue Engineering·Joseph D BerglundAthanassios Sambanis
Apr 19, 2005·The British Journal of Ophthalmology·H S DuaA Joseph
Apr 28, 2006·Investigative Ophthalmology & Visual Science·Yuwen LiuMay Griffith
Aug 8, 2006·Tissue Engineering·Wan-Ju LiRocky S Tuan
Sep 26, 2006·Tissue Engineering·Yaling ShiIvan Vesely
Sep 28, 2006·Investigative Ophthalmology & Visual Science·Andrew HopkinsonHarminder S Dua
Feb 27, 2007·Journal of Materials Science. Materials in Medicine·Maria NotaraJulie T Daniels
Jan 12, 2008·PloS One·Che J ConnonKeith M Meek
Mar 26, 2008·Molecular Reproduction and Development·Shengli MiZhongying Dou
Mar 24, 2009·The British Journal of Ophthalmology·C J ConnonK M Meek

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Citations

Sep 17, 2011·Tissue Engineering. Part a·Shengli MiChe John Connon
Dec 27, 2011·Tissue Engineering. Part C, Methods·Erik BraziulisErnst Reichmann
Nov 5, 2011·Regenerative Medicine·Sharon L MasonCarl M Sheridan
Sep 11, 2012·Advanced Drug Delivery Reviews·Ensanya A Abou NeelJung Keun Hyun
Nov 13, 2014·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·T FuchslugerB Bachmann
Dec 3, 2014·Journal of the Chinese Medical Association : JCMA·I-Lun TsaiYung-Hsin Cheng
Jun 12, 2012·Experimental Eye Research·Yun FengChe John Connon
Jan 21, 2016·Stem Cells International·Michel HaagdorensNadia Zakaria
Oct 22, 2013·Journal of Biomedical Materials Research. Part a·Bernice WrightChe J Connon
Jul 3, 2013·Journal of Biomedical Materials Research. Part a·Xianghua XiaoShengli Mi
Jul 7, 2011·Journal of Biomedical Materials Research. Part a·Shengli MiChe John Connon
May 24, 2011·Journal of Tissue Engineering and Regenerative Medicine·D KaramichosJ D Zieske
Jan 12, 2016·Journal of Biomedical Materials Research. Part a·Gengbo LiuVipuil Kishore
May 8, 2015·Tissue Engineering. Part C, Methods·Mark Ahearne, Amy P Lynch
Mar 6, 2012·Stem Cell Research·Roanne R JonesChe J Connon
Jan 11, 2014·Current Eye Research·Yaqing FengGerd Geerling
Aug 1, 2012·Drug Discovery Today·Bernice WrightChe J Connon
May 9, 2014·Journal of the Mechanical Behavior of Biomedical Materials·Siobhán E DunphyAlicia J El Haj
Mar 14, 2014·Tissue Engineering. Part a·Corinna PetschBjörn O Bachmann
Jun 19, 2016·International Journal of Biological Macromolecules·Shruthy KuttappanManitha B Nair
Apr 6, 2017·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·P Eberwein, T Reinhard
Jul 8, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Z XiaM Wei
Mar 10, 2017·Seminars in Immunology·Aaron H MorrisT R Kyriakides
Aug 15, 2018·Advanced Healthcare Materials·Hyun Jong LeeDavid Myung
May 31, 2018·Advanced Healthcare Materials·Rachelle N PalcheskoAdam W Feinberg
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Oct 12, 2012·Current Eye Research·Maria Fernanda ForniJosé Álvaro Pereira Gomes
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Courtney R LynchViness Pillay
Aug 8, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hyeonjun HongDong Sung Kim
Dec 7, 2018·Tissue Engineering. Part a·Joana WittStefan Schrader
Dec 29, 2020·Acta Ophthalmologica·Daniele SpinozziSilke Oellerich
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Xuan ZhaoJin Yuan
Feb 28, 2021·Journal of Biomedical Materials Research. Part a·A Hernández-Rangel, E San Martin-Martinez
Nov 13, 2017·Acta Biomaterialia·Jared L ZitnayJeffrey A Weiss
Jul 4, 2020·Experimental Eye Research·Tina B McKayDimitrios Karamichos
Apr 20, 2021·Experimental Eye Research·Andrey Yurevich AndreevSergey Petrovich Domogatsky

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