Plastic optical fiber-based biosensor platform for rapid cell detection

Biosensors & Bioelectronics
Gisele WandermurMarco Antônio Lemos Miguel

Abstract

This work presents a novel, fast response time, plastic optic fiber (POF) biosensor to detect Escherichia coli. It discloses the technique for the development, calibration and measurement of this robust and simple-to-construct POF biosensor. The probes in U-shaped format were manufactured with a specially developed device. The calibration process led to the evaluation of the sensitivity, accuracy and repeatability by using solutions of sucrose for obtaining refractive indices (RI) in the range 1.33-1.39 IR equivalent of water and bacteria, respectively. The POF probes were functionalized with antibody anti-E. coli serotype O55 and tested firstly with saline and then with bacterial concentrations of 10(4), 10(6), and 10(8) colony forming units/ml (CFU/ml). The optoelectronic setup consists of an 880 nm LED connected to the U-shaped probe driven by a sine waveform generated by the Simulink (from Matlab(®)). On the other side of the probe a photodetector generates a photocurrent which is amplified by a transconductance amplifier. The output voltage signal is read by the analog-to-digital (A/D) input of the microcontroller. In all tested concentrations, the results presented a tendency of a decrease in the output signal with time, ...Continue Reading

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Citations

Dec 13, 2016·Sensors·Omer Galip Saracoglu, Sekip Esat Hayber
Jun 27, 2015·Scientific Reports·Nianbing ZhongRong Chen
Mar 24, 2018·Journal of Biophotonics·Nurul H ZainuddinMohd H Yaacob
Dec 15, 2015·Advanced Materials·Amanda L Bole, Panagiotis Manesiotis

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