Plasticity of in vitro-generated urothelial cells for functional tissue formation

Tissue Engineering. Part a
Felix WezelJennifer Southgate

Abstract

Tissue-engineering and regenerative medicine strategies for the bladder and urinary tract are dependent on the ability to generate adequate numbers of differentiation-competent uro-epithelial cells. In situ, urothelium is a mitotically quiescent, but highly regenerative epithelium. Although evidence supports a resident, basally located urothelial progenitor population, no specific stem cell has been identified. Our aim was to isolate basal and suprabasal urothelial subpopulations and characterize their regenerative and differentiation potentials in vitro. We showed that the low-affinity nerve growth factor receptor (NGFR) is a cell surface-expressed marker that is restricted to basal cells in normal human and porcine urothelia in situ. We used NGFR immunoseparation and differential adherence to collagen to isolate subpopulations of urothelial cells for culture. Isolated basal-derived porcine NGFR⁺ urothelial cells initially showed a higher proliferative and clonogenic phenotype than their suprabasal NGFR⁻ counterparts in vitro. However, after a short period of adaptation to culture, both NGFR⁺ and NGFR⁻ subpopulations became indistinguishable and displayed similar long-term growth and differentiation potentials. Both population...Continue Reading

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Citations

Jul 6, 2014·Developmental Biology·Weilun SunEgbert Oosterwijk
Mar 11, 2017·Cell Death and Differentiation·Carl FishwickJennifer Southgate
May 23, 2019·Journal of Tissue Engineering and Regenerative Medicine·Clara Ibel ChamorroMagdalena Fossum
Feb 6, 2017·Journal of Tissue Engineering and Regenerative Medicine·Stéphane ChabaudStéphane Bolduc
Mar 31, 2018·Nature Reviews. Urology·Cagatay GünesChristian Bolenz
Apr 4, 2021·International Journal of Molecular Sciences·Felix WezelCagatay Günes
Jun 15, 2021·Frontiers in Cellular and Infection Microbiology·Benjamin O MurrayJennifer L Rohn
Sep 7, 2021·Stem Cells and Development·Monika BuhlMarta Pokrywczynska

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Methods Mentioned

BETA
fluorescence-activated cell sorting
flow cytometry

Software Mentioned

Clono
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