Islet Schwann (glial) cells and pericytes are the microorgan's accessory cells positioned at the external and internal boundaries facing the exocrine pancreas and endothelium, respectively, adjacent to the endocrine cells. Plasticity of glial cells and pericytes is shown in the glial scar formation after injury to the central nervous system. It remains unclear whether similar reactive cellular responses occur in insulitis. We applied three-dimensional (3D) histology to perform qualitative and quantitative analyses of the islet Schwann cell network and pericytes in normal, streptozotocin-injected (positive control of gliosis) and NOD mouse models. Vessel painting paired with immunostaining of mouse pancreatic tissue was used to reveal the islet Schwann cells and pericytes and their association with vasculature. Transparent islet specimens were prepared by optical clearing to facilitate 3D confocal microscopy for panoramic visualisation of the tissue networks. In-depth microscopy showed that the islet Schwann cell network extends from the peri-islet domain into the core. One week after streptozotocin injection, we observed intra-islet perivascular gliosis and an increase in pericyte density. In early/moderate insulitis in the NOD...Continue Reading
Antigen presentation in the peripheral nervous system: Schwann cells present endogenous myelin autoantigens to lymphocytes
Ultrastructural evidence for the presence of a glial sheath investing the islets of Langerhans in the pancreas of mammals
Intercellular adhesion molecule-1 (ICAM-1) expression in the islets of the non-obese diabetic and low-dose streptozocin-treated mouse
The endocrine pancreas of spontaneously diabetic db/db mice: microangiopathy as revealed by transmission electron microscopy
Islet injury induces neurotrophin expression in pancreatic cells and reactive gliosis of peri-islet Schwann cells
Adhesion molecules and microvascular changes in the nonobese diabetic (NOD) mouse pancreas. An NO-inhibitor (L-NAME) is unable to block adhesion inflammation-induced activation
Enterocolitis induced by autoimmune targeting of enteric glial cells: a possible mechanism in Crohn's disease?
Ultrastructure of islet microcirculation, pericytes and the islet exocrine interface in the HIP rat model of diabetes.
Microtome-free 3-dimensional confocal imaging method for visualization of mouse intestine with subcellular-level resolution.
The role of blood vessels, endothelial cells, and vascular pericytes in insulin secretion and peripheral insulin action.
Unexpected acceleration of type 1 diabetes by transgenic expression of B7-H1 in NOD mouse peri-islet glia.
Optical clearing facilitates integrated 3D visualization of mouse ileal microstructure and vascular network with high definition
Vagal nerve stimulation protects against burn-induced intestinal injury through activation of enteric glia cells.
Three-dimensional optical method for integrated visualization of mouse islet microstructure and vascular network with subcellular-level resolution
Optical clearing improves the imaging depth and signal-to-noise ratio for digital analysis and three-dimensional projection of the human enteric nervous system.
3-D illustration of network orientations of interstitial cells of Cajal subgroups in human colon as revealed by deep-tissue imaging with optical clearing.
3-D imaging and illustration of the perfusive mouse islet sympathetic innervation and its remodelling in injury.
3-D imaging, illustration, and quantitation of enteric glial network in transparent human colon mucosa
3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected
3-D Imaging Reveals Participation of Donor Islet Schwann Cells and Pericytes in Islet Transplantation and Graft Neurovascular Regeneration
3-D imaging of islets in obesity: formation of the islet-duct complex and neurovascular remodeling in young hyperphagic mice
Surface coating of pancreatic islets with neural crest stem cells improves engraftment and function after intraportal transplantation
PanIN-associated pericyte, glial, and islet remodeling in mice revealed by 3D pancreatic duct lesion histology
A "Clearer" View of Pancreatic Pathology: A Review of Tissue Clearing and Advanced Microscopy Techniques
Peripheral nerve grafts implanted into the substantia nigra in patients with Parkinson's disease during deep brain stimulation surgery: 1-year follow-up study of safety, feasibility, and clinical outcome
Three-dimensional islet graft histology: panoramic imaging of neural plasticity in sympathetic reinnervation of transplanted islets under the kidney capsule
Naturally presented HLA class I-restricted epitopes from the neurotrophic factor S100-β are targets of the autoimmune response in type 1 diabetes
Recruited fibroblasts reconstitute the peri-islet membrane: a longitudinal imaging study of human islet grafting and revascularisation
Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
Recapitulating pancreatic cell-cell interactions through bioengineering approaches: the momentous role of non-epithelial cells for diabetes cell therapy.
Identification of astroglia-like cardiac nexus glia that are critical regulators of cardiac development and function.
Blood Brain Barrier
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