Plasticizer Bis(2-ethylhexyl) Phthalate Causes Meiosis Defects and Decreases Fertilization Ability of Mouse Oocytes in Vivo

Journal of Agricultural and Food Chemistry
Zhenzhen LuJianmin Su

Abstract

Bis(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer in polyvinyl chloride (PVC) plastics. Humans and animals are widely and continuously exposed to DEHP, especially with respect to diet, which is associated with reproductive diseases. Nevertheless, the effects and underlying mechanisms of DEHP exposure on oocytes in vivo remain ambiguous. In this study, we found that oral administration of DEHP (40 μg/kg body weight per day for 14 days) markedly reduced the maturation and fertilization of oocytes in vivo. In addition, DEHP caused oxidative stress, increased reactive oxygen species generation, promoted early apoptosis, and resulted in DNA damage in mouse oocytes. Moreover, DEHP exposure caused mitochondrial damage, reduced ATP content, down-regulated actin expression, and disturbed the spindle assembly and chromosome alignment in mouse oocytes. Furthermore, DEHP exposure remarkably impaired the localization and protein level of Juno, the sperm receptor on the membrane of oocytes. The levels of DNA methylation, H3K9me3, and H3K9ac were also altered in the DEHP-exposed mouse oocytes. Thus, our results indicated that DEHP exposure reduced the maturation and fertilization capabilities of mouse oocytes by affecting cytosk...Continue Reading

Citations

Sep 24, 2020·International Journal of Environmental Research and Public Health·Henrieta HlisníkováAlexander Sirotkin
Sep 1, 2020·Environmental Pollution·Ming-Yu ZhangShun-Feng Cheng
Jan 8, 2021·Biology of Reproduction·Kelli F Malott, Ulrike Luderer
Feb 22, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hyekyoung Hannah You, Gwonhwa Song
Jul 10, 2021·Best Practice & Research. Clinical Endocrinology & Metabolism·Eleftheria M PanagiotouPauliina Damdimopoulou
Jul 4, 2021·The Science of the Total Environment·Yimeng TianXin Zhang
Aug 16, 2021·Ecotoxicology and Environmental Safety·Si-Cheng ZhaoYu Liu

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