Abstract
Bis(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer in polyvinyl chloride (PVC) plastics. Humans and animals are widely and continuously exposed to DEHP, especially with respect to diet, which is associated with reproductive diseases. Nevertheless, the effects and underlying mechanisms of DEHP exposure on oocytes in vivo remain ambiguous. In this study, we found that oral administration of DEHP (40 μg/kg body weight per day for 14 days) markedly reduced the maturation and fertilization of oocytes in vivo. In addition, DEHP caused oxidative stress, increased reactive oxygen species generation, promoted early apoptosis, and resulted in DNA damage in mouse oocytes. Moreover, DEHP exposure caused mitochondrial damage, reduced ATP content, down-regulated actin expression, and disturbed the spindle assembly and chromosome alignment in mouse oocytes. Furthermore, DEHP exposure remarkably impaired the localization and protein level of Juno, the sperm receptor on the membrane of oocytes. The levels of DNA methylation, H3K9me3, and H3K9ac were also altered in the DEHP-exposed mouse oocytes. Thus, our results indicated that DEHP exposure reduced the maturation and fertilization capabilities of mouse oocytes by affecting cytosk...Continue Reading
Citations
May 2, 2019·Molecular Human Reproduction·Pengxiang QuYongsheng Wang
Feb 13, 2020·Reproductive Sciences·Quanli AnJianmin Su
Sep 25, 2019·Cells·Yan-Li SunQing-Yuan Sun
Aug 8, 2019·Archives of Toxicology·Qi-Long HeShu-Zhen Liu
Jan 10, 2020·PLoS Genetics·Luciann CuencaMónica P Colaiácovo
Sep 24, 2020·International Journal of Environmental Research and Public Health·Henrieta HlisníkováAlexander Sirotkin
Apr 27, 2019·Frontiers in Genetics·Xiaoyuan LinLuc Peelman
Oct 9, 2020·Animal Reproduction·Zvi RothDorit Kalo
Dec 2, 2020·Environmental Pollution·Lin-Jing WuNa Guo
Sep 1, 2020·Environmental Pollution·Ming-Yu ZhangShun-Feng Cheng
Mar 7, 2020·Environmental Pollution·Zhi-Ming DingLi-Jun Huo
Jan 7, 2021·Reproduction in Domestic Animals = Zuchthygiene·Tong HaoXueming Zhao
Jan 28, 2021·Environmental Health Perspectives·Nolwenn AdamSakina Mhaouty-Kodja
Jan 8, 2021·Biology of Reproduction·Kelli F Malott, Ulrike Luderer
Feb 22, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hyekyoung Hannah You, Gwonhwa Song
Mar 28, 2021·Toxicology·Peipei QiaoJianmin Su
Nov 2, 2020·Reproductive Toxicology·Emily BrehmJodi A Flaws
May 5, 2021·Reproduction, Fertility, and Development·Haiying WangYu Deng
Sep 12, 2020·Ecotoxicology and Environmental Safety·Xianlei JiangJianmin Su
Jul 10, 2021·Best Practice & Research. Clinical Endocrinology & Metabolism·Eleftheria M PanagiotouPauliina Damdimopoulou
Jul 4, 2021·The Science of the Total Environment·Yimeng TianXin Zhang
Aug 16, 2021·Ecotoxicology and Environmental Safety·Si-Cheng ZhaoYu Liu