PMID: 3383847Jun 1, 1988Paper

Plastid-localised seed acyl-carrier protein of Brassica napus is encoded by a distinct, nuclear multigene family

European Journal of Biochemistry
R SaffordS G Hughes

Abstract

Acyl-carrier protein (ACP) is a key component involved in the regulation of fatty acid biosynthesis in plants. cDNA clones encoding ACP from Brassica napus (oil seed rape) embryos have been isolated using oligonucleotide probes derived from heterologous ACPs. Analysis of the DNA sequence data, in conjunction with N-terminal amino acid sequence data, revealed ACP to be synthesized from nuclear DNA as a precursor containing a 51-amino-acid N-terminal extension. Immunocytochemical studies showed ACP to be localised solely within the plastids of B. napus seed tissue and it would therefore appear that the N-terminal extension functions as a transit peptide to direct ACP into these organelles. Analysis of several cDNA clones revealed sequence heterogeneity and thus evidence for an ACP multigene family. From ten cDNA clones, six unique genes, encoding five different mature ACP polypeptides, were identified. Northern blot hybridisation studies provide evidence that the seed and leaf forms of rape ACP are encoded by structurally distinct gene sets.

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Citations

May 1, 1992·Plant Molecular Biology·A R Slabas, T Fawcett
Nov 1, 1990·Plant Molecular Biology·K M Schmid, J B Ohlrogge
Oct 1, 1991·Plant Molecular Biology·A R SlabasC M Sidebottom
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Carl AndreJohn Shanklin
Feb 25, 1989·Nucleic Acids Research·M A Post-BeittenmillerJ B Ohlrogge
Jan 1, 1992·Plant Physiology·A Hlousek-RadojcićJ B Ohlrogge
Nov 13, 1990·European Journal of Biochemistry·J E FroehlichJ G Jaworski
Jan 10, 2014·Molecular Plant-microbe Interactions : MPMI·Jun WangGary Stacey
Oct 1, 1991·Molecular & General Genetics : MGG·L Hansen, P von Wettstein-Knowles
Jan 18, 2015·Chemico-biological Interactions·Hao ZhangYuanhua Yu
Feb 26, 1991·Biochimica Et Biophysica Acta·J B OhlroggeC R Somerville
Feb 26, 1990·FEBS Letters·Y Gavel, G von Heijne
Oct 1, 1989·European Journal of Biochemistry·J G JaworskiJ B Ohlrogge
May 31, 1996·Biochimica Et Biophysica Acta·J L Harwood
Apr 8, 1992·Biochimica Et Biophysica Acta·P S SheldonA R Slabas
Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek
Apr 5, 1991·Science·C Somerville, J Browse

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