Plastoquinol generates and scavenges reactive oxygen species in organic solvent: Potential relevance for thylakoids

Biochimica Et Biophysica Acta. Bioenergetics
Sergey A Khorobrykh, Esa Tyystjärvi

Abstract

The present work reports reactions of plastoquinol (PQH2-9) and plastoquinone (PQ-9) in organic solvents and summarizes the literature to understand similar reactions in thylakoids. In thylakoids, PQH2-9 is oxidized by the cytochrome b6/f complex (Cyt b6/f) but some PQH2-9 is also oxidized by reactions in which oxygen acts as an electron acceptor and is converted to reactive oxygen species (ROS). Furthermore, PQH2-9 reacts with ROS. Light enhances oxygen-dependent oxidation of PQH2-9. We examined the oxidation of PQH2-9 via dismutation of PQH2-9 and PQ-9 and scavenging of the superoxide anion radical (O2-) and hydrogen peroxide (H2O2) by PQH2-9. Oxidation of PQH2-9 via dismutation to semiquinone was slow and independent of pH in organic solvents and in solvent/buffer systems, suggesting that intramembraneous oxidation of PQH2-9 in darkness mainly proceeds via reactions catalyzed by the plastid terminal oxidase and cytochrome b559. In the light, oxidation of PQH2-9 by singlet oxygen and by O2- formed in PSI contribute significantly. In addition, Cyt b6/f forms H2O2 with a PQH2-9 dependent mechanism. Measurements of the reaction of O2- with PQH2-9 and PQ-9 in acetonitrile showed that O2- oxidizes PQH2-9, forming PQ-9 and several ...Continue Reading

Associated Datasets

Citations

Sep 5, 2018·FEBS Letters·Maria M Borisova-MubarakshinaBoris N Ivanov
Feb 2, 2019·Physiologia Plantarum·Maria M Borisova-MubarakshinaBoris N Ivanov
Sep 19, 2019·FEBS Letters·Sergey KhorobrykhEsa Tyystjärvi
Jan 16, 2020·Plants·Sergey KhorobrykhEsa Tyystjärvi
Sep 6, 2020·The Plant Journal : for Cell and Molecular Biology·Heta MattilaEsa Tyystjärvi
Sep 21, 2018·International Journal of Molecular Sciences·Martin ČernýBřetislav Brzobohatý
Apr 4, 2021·International Journal of Molecular Sciences·Beatrycze NowickaRenata Szymańska
Jul 28, 2020·Trends in Plant Science·Michel Havaux

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