Platelet activating factor degradation in tear fluid from guinea pigs with allergic conjunctivitis

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
M KatoK Nakata

Abstract

The purpose of this study was to investigate the role of platelet-activating factor (PAF) and PAF acetylhydrolase (AH) in conjunctiva. The influence of PAF on conjunctival vascular permeability and the presence of PAF or its metabolites in tears from guinea pigs with allergic conjunctivitis were investigated. We instilled PAF to the eyes of guinea pigs and evaluated vascular permeability. Tear samples were collected from passively sensitized guinea pigs, and the concentration of PAF and its metabolites determined by liquid chromatography-tandem mass spectrometry. Exogenous PAF degradation in tear samples was evaluated with or without diisopropyl fluorophosphate (DFP). Topically applied PAF increased vascular permeability in conjunctiva. In the tear samples from guinea pigs with allergic conjunctivitis, PAF could not be detected. However, 40 +/- 6 ng/ml of lyso-platelet activating factor (lyso-PAF) and 230 +/- 50 ng/ml of 1-alkyl-2-acyl-sn-glycero-3-phosphocholine were detected at 10 min after challenge. Exogenous PAF was rapidly degraded in the tear samples from guinea pigs with allergic conjunctivitis, but not from normal guinea pigs. This PAF degradation was inhibited by DFP. These results suggest that PAF in the tear fluid i...Continue Reading

References

Jan 1, 1990·Journal of Ocular Pharmacology·A MullerC Bonne
Jan 1, 1990·International Archives of Allergy and Applied Immunology·C TettaG Camussi
Jun 11, 1996·Biochimica Et Biophysica Acta·D M StafforiniT M McIntyre
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Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Sep 11, 2003·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Masatomo KatoSuguru Miyaji
Nov 2, 2006·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Naoki OkumuraHisayuki Ueno
Nov 18, 2003·Allergy·D A GronebergU Wahn
Oct 28, 2005·Current Eye Research·Naoki OkumuraHisayuki Ueno

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