PMID: 9443941Jan 28, 1998Paper

Platelet-activating factor induction of activator protein-1 signaling in bronchial epithelial cells

Molecular Pharmacology
T D LeVanM Halonen

Abstract

Platelet-activating factor (PAF) has been implicated in the pathogenesis of allergic and inflammatory events in the airway. In the present study, we sought to determine if PAF receptors are present on human bronchial epithelial cells and whether PAF binding to these receptors leads to activation of activator protein-1 (AP-1)-mediated transcription. Radioligand binding studies demonstrated specific binding sites for the PAF antagonist [3H]WEB 2086 (3-[4-(2-chlorophenyl)-9-methyl-6H-thieno[3,2-f]-[1,2,4]triazolo[4,3- a][1,4]diazepine-2-yl]-1-(4-morpholinyl)-1-propanone) on primary bronchial epithelial cells with an equilibrium dissociation constant (Kd) = 9.8 nM and maximal density of binding sites (Bmax) = 42.4 fmol/mg of protein. The expression of PAF receptors in these cells was further confirmed by reverse transcriptase-polymerase chain reaction, which revealed amplification products derived from PAF receptor mRNA corresponding to transcripts 1 and 2. In the bronchial epithelial cell line BEAS-2B transfected with an expression plasmid for the human PAF receptor, PAF stimulation increased AP-1 DNA binding activity as determined by electrophoretic mobility shift assays. The Fos and Jun family proteins were identified as compone...Continue Reading

References

Oct 15, 1991·Biochemical and Biophysical Research Communications·R D YeC G Cochrane
Mar 1, 1991·The Journal of Allergy and Clinical Immunology·M Chan-YeungH Salari
Sep 1, 1990·American Journal of Respiratory Cell and Molecular Biology·J GomezM Halonen
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Apr 30, 1985·Biochemical and Biophysical Research Communications·S B HwangT Y Shen
Oct 1, 1995·American Journal of Respiratory Cell and Molecular Biology·S L CamhiA M Choi
Jun 23, 1995·The Journal of Biological Chemistry·V V KravchenkoR D Ye
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H E BazanN G Bazan
Mar 1, 1993·American Journal of Respiratory Cell and Molecular Biology·P B ChaseJ W Regan
Dec 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L Kuitert, N C Barnes
May 29, 2013·Molecular Systems Biology·Mathieu CourcellesPierre Thibault

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Citations

Jun 18, 2002·Immunopharmacology and Immunotoxicology·Chun Kwok WongChristopher Wai Kei Lam
Aug 27, 2013·Molecular and Cellular Biochemistry·Astrid J FeuerhermBerit Johansen
Apr 14, 2004·Critical Reviews in Clinical Laboratory Sciences·Chun Kwok WongChristopher Wai Kei Lam
Jul 13, 2021·The Journal of Sexual Medicine·Skyler HowellMohit Khera

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