Platelet activating factor inhibits the expression of matrix metalloproteinases and affects invasiveness and differentiation in a system of human neuroblastoma clones

Biological Chemistry
Emanuela BarlettaSalvatore Ruggieri

Abstract

Platelet Activating Factor (PAF), an inflammatory bioactive lipid, has been shown to be involved in the regulation of the activity of matrix metalloproteinases (MMPs). In view of the role played by MMPs in tumor cell invasiveness, we investigated whether PAF influences MMP activity in a system of neuroblastoma clones, the AA5 and AE12 cells, isolated from the human LaN1 neuroblastoma cell line. These clones were characterized by an inverse relationship between invasiveness and differentiative capacity and by the expression of specific cell surface PAF receptors. We found that the levels of mRNAs specific for MMP-2 and for MT1-MMP, the MMP-2 activator, were reduced in both clones treated with 300 nM PAF. These changes are consistent with the reduced secretion and activation of MMP-2 found in the neuroblastoma clones exposed to PAF. These effects were accompanied by an inhibition of invasiveness through Matrigel and by a promotion of differentiation, as revealed by an increased percentage of cells with neurites. The finding that both neuroblastoma clones exposed to the metalloproteinase inhibitors, BB3103 and 1,10-phenanthroline, increased their differentiative capacity and reduced their invasiveness through Matrigel, represents ...Continue Reading

References

Dec 1, 1990·Molecular and Cellular Biology·S M Frisch, J H Morisaki
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F CarmichaelG P Stricklin
Jan 1, 1987·Molecular Neurobiology·A D Lander
Mar 4, 1997·International Journal of Cancer. Journal International Du Cancer·E BarlettaS Ruggieri
Aug 1, 1997·Brain Research. Molecular Brain Research·A NegroS Garbisa
Dec 5, 1998·Journal of Immunological Methods·J M WangS Su
Jan 12, 2000·Oncogene·C S WilliamsR N DuBois
Mar 31, 2000·Molecular Medicine Today·L J McCawley, L M Matrisian
Aug 26, 2000·Science·M J Galko, M Tessier-Lavigne
Aug 31, 2000·Annual Review of Biochemistry·S M PrescottT M McIntyre
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E RovidaP Dello Sbarba
Feb 13, 2001·Critical Reviews in Oncology/hematology·R Giavazzi, G Taraboletti
Feb 13, 2001·Current Biology : CB·L J McCawley, L M Matrisian

❮ Previous
Next ❯

Citations

Aug 28, 2007·Cancer Metastasis Reviews·Vladislava Melnikova, Menashe Bar-Eli
May 6, 2014·Experimental Neurology·Erica SarchielliGabriella B Vannelli
Aug 1, 2006·Cancer Genetics and Cytogenetics·Yu HuTang-chun Wu
Jun 7, 2016·Biochimica Et Biophysica Acta·Ihsan Ekin DemirGüralp O Ceyhan
Jan 15, 2013·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Seiichi OdateMitsuo Katano
Feb 18, 2011·The Journal of General Virology·Anna GoldaKrzysztof Pyrc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.