PMID: 11910521Mar 23, 2002Paper

Platelet-activating factor (PAF) enhances apoptosis induced by ultraviolet radiation in corneal epithelial cells through cytochrome c-caspase activation

Current Eye Research
X Ma, H E Bazan

Abstract

To examine the role of platelet-activating factor (PAF) on apoptosis of corneal epithelial cells exposed to radiation. Rabbit corneal epithelial (RCE) and human corneal epithelial (HCE) cells were exposed to UVC radiation and then to carbamyl PAF (cPAF) for different increments of time. The PAF antagonist BN50739 was added 30 min before cPAF. The caspase inhibitors Ac-DEVD-CHO and Ac-YVAD-CHO were added 1 h before, and the phospholipase A(2) (PLA(2)) inhibitor MAFP was added 3 h before UVC irradiation. FITC-dUTP TUNEL and DAPI staining were performed to assess the percentage of apoptotic cells. DNA ladder analysis was used to investigate apoptosis induced by different intensities of UVC (50-600 J/m(2)) with or without cPAF. Caspase activation and release of cytochrome c from mitochondria to cytosol were determined by Western blot. While only 2.7% of RCE cells were DAPI positive in controls incubated for 12 h, 44% of cells were stained positive 4 h after irradiation; these values increased to 63% in the presence of cPAF. Cells incubated with cPAF alone were similar to controls. TUNEL staining and DNA laddering showed also increased in apoptosis after PAF treatment of UV-irradiated cells and BN50739 blocked the effect of cPAF. cP...Continue Reading

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Citations

Dec 8, 2009·Journal of Lipid Research·Sachidananda Kenchegowda, Haydee E P Bazan
Nov 22, 2012·Journal of Cellular Physiology·Ivana SirangeloMaria Luisa Balestrieri
Oct 1, 2004·The Journal of Biological Chemistry·Fanny BoninSteffany A L Bennett
Jan 31, 2014·The Journal of Biological Chemistry·Johanna RiveraDavid L Goldman
Jan 8, 2004·Critical Reviews in Clinical Laboratory Sciences·Diana M StafforiniStephen M Prescott
Aug 28, 2007·Cancer Metastasis Reviews·Vladislava Melnikova, Menashe Bar-Eli
Nov 23, 2017·Vestnik oftalmologii·S E Avetisov, M N Narbut
May 1, 2010·Molecular Neurobiology·Salomon Esquenazi, Haydee E P Bazan
May 17, 2008·Cancer Research·Coimbatore S SreevidyaStephen E Ullrich
Dec 29, 2020·Prostaglandins & Other Lipid Mediators·Barbara DalmasoCarolina Beltrame Del Debbio
Sep 5, 2002·Progress in Retinal and Eye Research·Haydee Bazan, Paulo Ottino

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