Platelet activation at the onset of human endotoxemia is undetectable in vivo

Platelets
Waltraud Cornelia SchrottmaierAlice Assinger

Abstract

Infection induces platelet activation and consumption, which leads to thrombocytopenia, enhances microvascular thrombosis, impairs microcirculation and eventually triggers disseminated intravascular coagulation (DIC). It is well characterized that endotoxemia results in a pro-inflammatory and pro-coagulatory state, which favors platelet activation. However the early, direct effects of endotoxemia on platelets have not been investigated so far. Therefore we aimed to determine the early effects of the endotoxin lipopolysaccharide (LPS) on platelet function in vivo. In a human endotoxemia model, 15 healthy volunteers were stimulated with LPS (2 ng/kg). Blood was drawn before, 10, 30 and 60 min after LPS challenge and platelet activation analyzed by flow cytometry (GPIIb/IIIa activation, surface CD62P and CD40L, intraplatelet reactive oxygen formation and platelet-leukocyte aggregates) and ELISA (sCD40L, sCD62P and CXCL4). In parallel, blood samples and platelets were spiked with LPS (50 pg/ml) in vitro and monitored over 60 min for the same platelet activation markers. In vitro platelet stimulation with LPS activated platelets independent of the presence of leukocytes and enhanced their adhesion to endothelial cells. In contrast, ...Continue Reading

References

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Apr 24, 2015·Thrombosis and Haemostasis·Waltraud C SchrottmaierAlice Assinger

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Citations

Nov 20, 2016·Clinical Pharmacology and Therapeutics·P MatznellerM Zeitlinger
Mar 14, 2018·Platelets·Hind Hamzeh-CognasseFabrice Cognasse
Aug 31, 2019·Clinical Oral Investigations·Markus LakyAlice Assinger
Aug 6, 2016·Alternatives to Laboratory Animals : ATLA·Stefan FennrichSandra Stoppelkamp
Apr 27, 2021·British Journal of Anaesthesia·Matthew B A HarmonNicole P Juffermans

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