PMID: 9163596May 1, 1997Paper

Platelet activation by a novel solid-phase agonist: effects of VWF immobilized on polystyrene beads

British Journal of Haematology
M W StewartA R Shaw

Abstract

The interaction between platelets stirred in suspension and VWF immobilized on polystyrene beads was studied. Platelets aggregated and released ATP in response to stirring with VWF beads. Closer examination of the interaction using transmission electron microscopy revealed that the platelets did not simply aggregate with one another but initially adhered to the beads and spread. Platelets in suspension then bound to the bead-adherent platelets forming layers of platelets associated with each bead. The VWF bead-induced platelet activation was completely inhibited by addition of monoclonal antibody (mAb) to GPIb or GPIIb/IIIa. In addition, the activation response was inhibited in the presence of aspirin, indomethacin or the thromboxane receptor antagonist BM13.177, demonstrating a dependence on an intact cyclo-oxygenase pathway. Platelet function studies were carried out on 30 patients with a history of mild bleeding using conventional optical aggregation and VWF bead-induced platelet activation. 12 patients were abnormal by conventional optical aggregometry, whereas 27 patients showed depressed ATP release in response to VWF beads. The results suggest that easily-bruised patients may have a platelet function defect rather than a...Continue Reading

Citations

Apr 18, 2002·Thrombosis Research·Chitra KrishnamurtiStephen W Rothwell
Oct 30, 2001·British Journal of Pharmacology·A RadomskiM W Radomski
Sep 2, 1998·Journal of Biomaterials Science. Polymer Edition·H Nygren, M Broberg

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