Platelet adhesion and activation on polyethylene glycol modified polyurethane surfaces. Measurement of cytoplasmic calcium

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
K D ParkT Okano

Abstract

Polyurethane (PU) surfaces were modified by coupling polyethylene glycol (PEG; molecular weight, 1,000) chains carrying different terminal groups (PU-PEG1K-OH, PU-PEG1K-NH2, PU-PEG1K-SO3) and longer PEG chains (MW, 3,350; PU-PEG3.4K-OH). The modified PU surfaces have the same PEG (1K) chain density. Surface induced platelet activation was evaluated by measuring cytoplasmic free calcium concentration in platelets contacting modified surfaces, and platelet adhesion onto modified surfaces was investigated in vitro. Cytoplasmic free calcium levels in platelets contacting PU-PEG-SO3 remained relatively constant, in contrast to the significant increase observed for PU-PEG-NH2, PU-PEG-OH, and control PU surfaces. The degree of platelet adhesion clearly demonstrates that all PEG graft surfaces prevented platelet adhesion. Among PEG1K surfaces, PU-PEG-SO3 shows the lowest platelet adhesion. In the case of relatively longer PEG grafted surfaces (PU-PEG3.4K-OH and PU-PEG3.4K-Hep), both surfaces were found to prevent the increase in both cytoplasmic free calcium and platelet adhesion. These results suggest that longer PEG chain grafting is more effective than shorter grafting in preventing platelet activation and adhesion because of the hi...Continue Reading

Citations

Mar 28, 2009·Journal of the Royal Society, Interface·Kenichi NagaseTeruo Okano
Jun 8, 2007·The Review of Scientific Instruments·Yves MartinPatrick Vermette
May 29, 2016·Biointerphases·Brooke L FarrugiaMegan S Lord
Feb 9, 2002·Journal of Biomedical Materials Research·Hyung Dal ParkNobuhiko Yui
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Hyung Dal ParkNobuhiko Yui
Dec 18, 2002·Journal of Biomaterials Science. Polymer Edition·Won Kyu LeeNobuhiko Yui
Mar 3, 2019·International Journal of Molecular Sciences·Iolanda FrancoliniAntonella Piozzi
Aug 5, 2004·The International Journal of Artificial Organs·M C AmoureuxS Szathmary

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