Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility

International Journal of Implant Dentistry
Masayuki NakamuraTomoyuki Kawase

Abstract

Platelet-rich plasma (PRP) is often used to improve surface biocompatibility. We previously found that platelets rapidly adhere to plain commercially pure titanium (cp-Ti) plates in the absence, but not in the presence, of plasma proteins. To further expand on these findings, in the present study, we switched titanium plates from a plain surface to a rough surface that is blasted with calcium phosphate (CaP) powder and then examined platelet adhesion and activation. Elemental distribution in CaP-blasted cp-Ti plates was analyzed using energy-dispersive X-ray spectroscopy. PRP samples prepared from anticoagulated blood samples of six healthy, non-smoking adult male donors were loaded on CaP-blasted cp-Ti plates for 1 h and fixed for examination of platelet morphology and visualization of PDGF-B and platelet surface markers (CD62P, CD63) using scanning electron microscopy and fluorescence microscopy. Plain SUS316L stainless steel plates used in injection needles were also examined for comparison. Significant amounts of calcium and phosphate were detected on the CaP-blasted cp-Ti surface. Platelets rapidly adhered to this surface, leading to higher activation. Platelets also adhered to the plain stainless surface; however, the lev...Continue Reading

References

Jul 1, 1997·Journal of Biomedical Materials Research·K IshikawaK Asaoka
Jun 5, 1998·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·K de GrootJ A Jansen
Dec 4, 1998·The Journal of Oral Implantology·D R VillarrealJ L Ong
Sep 16, 2004·Journal of Biomedical Materials Research. Part a·Christine KnabeHala Zreiqat
Aug 19, 2007·Biomaterials·John E Davies
Dec 25, 2007·Journal of Materials Science. Materials in Medicine·E A dos SantosK Anselme
May 20, 2009·Blood Reviews·Price Blair, Robert Flaumenhaft
Jun 6, 2009·PloS One·Sidney OmelonMarc D Grynpas
Aug 12, 2009·Clinical Oral Implants Research·Rüdiger JunkerJohn A Jansen
Nov 18, 2010·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Elizaveta KonMaurilio Marcacci
Jul 31, 2012·Biologicals : Journal of the International Association of Biological Standardization·Mito KobayashiHiromasa Yoshie
Apr 16, 2015·Clinical Implant Dentistry and Related Research·Taisuke WatanabeHayato Ohshima
May 16, 2015·International Journal of Laboratory Hematology·R J TraversJ H Morrissey
Jul 20, 2018·Scientific Reports·Francesco RobottiAldo Ferrari
Aug 2, 2018·International Journal of Implant Dentistry·Toshihisa ToyodaTomoyuki Kawase
Aug 18, 2018·Journal of Tissue Engineering·Laila DamiatiMatthew J Dalby
Oct 3, 2018·International Journal of Implant Dentistry·Yutaka KitamuraTomoyuki Kawase
Jan 25, 2019·Biomaterials Research·Jiwoon JeongChan Yeong Heo
May 21, 2019·Frontiers in Medicine·Reiner K W MailerThomas Renné
Feb 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Daniel Arcos, María Vallet-Regí
Aug 6, 2020·Bioengineering·Tomoyuki KawaseCarlos Fernando Mourão
Jul 10, 2017·ACS Biomaterials Science & Engineering·Ana CivantosAnder Abarrategi

❮ Previous
Next ❯

Citations

Jan 27, 2021·International Journal of Molecular Sciences·Atsushi SatoTomoyuki Kawase

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
scanning electron microscopy
atomic force microscopy
AFM

Software Mentioned

WinROOF
Systat
SigmaPlot

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.