PMID: 8963717May 1, 1996Paper

Platelet adhesion to multimeric and dimeric von Willebrand factor and to collagen type III preincubated with von Willebrand factor

Arteriosclerosis, Thrombosis, and Vascular Biology
Y P WuJ J Sixma

Abstract

As part of a systematic study of platelet interaction with adhesive proteins under flow conditions, we studied platelet adhesion to multimeric and dimeric von Willebrand factor (vWF) coated to glass. vWF-dependent adhesion to collagen type III was studied for comparison. Adhesion to glass-coated vWF and vWF-mediated adhesion to collagen type III were in many respects similar. Both showed no decrease at increasing shear rates and a decline to 50% of maximum with a low-molecular-weight multimeric fraction. Adhesion to glass-coated vWF was partially inhibited by heparin and completely inhibited by prostaglandin I(2) and anti-glycoprotein (GP) Ib and anti-GPIIb-IIIa antibodies. vWF-dependent adhesion to collagen was not inhibited by heparin, was partially inhibited by anti-GPIIb-IIIa, and was completely inhibited by prostaglandin I(2) and anti-GPIb. Recombinant dimeric vWF was made by deletion of the propeptide and expression in Chinese hamster ovary cells. Adhesion was 50% of that with plasma vWF, and larger concentrations of dimeric vWF were required. Adhesion to dimeric vWF was optimal at 1500 s(-1), with a gradual decrease at higher shear rates. We conclude that adhesion to collagen type III is strongly but not completely deter...Continue Reading

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Citations

Jul 1, 1990·British Journal of Haematology·P G de Groot, J J Sixma
Nov 17, 2004·Thrombosis Journal·Robert H Williams, Matthias U Nollert
Jul 17, 2015·Scientific Reports·Jonathan CowmanDermot Kenny
Nov 15, 2014·Blood·Shuju FengVahid Afshar-Kharghan
May 11, 2002·Photochemistry and Photobiology·Patrick FungaloiGlenn LaMuraglia
Apr 23, 2004·Journal of Thrombosis and Haemostasis : JTH·R W FarndaleP G de Groot
Oct 23, 2015·American Journal of Hematology·Ciprian B AneaJulia E Brittain
Apr 29, 2003·British Journal of Haematology·Erim Van OsGijsbert Van Willigen
Jan 25, 2002·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·T NishiyaY Ikeda

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