PMID: 9423814Jan 10, 1998Paper

Platelet aggregates as markers of platelet activation: characterization of flow cytometric method suitable for clinical applications

American Journal of Hematology
W JyY S Ahn

Abstract

The present paper describes a flow cytometric method for assay of platelet aggregates (PAg) in blood. This method combines and simplifies previously reported techniques, simultaneously enumerating PAg formed upon platelet activation, their expression of activation marker CD62P (P-selectin), and their content of bound leukocytes (LPAg). The sensitivity of this method to low levels of agonists (ADP, collagen) is compared to conventional aggregometry and some features of platelet-leukocyte interaction are explored. The results were: (1) ADP or collagen induced a dose-dependent increase in PAg number and corresponding decline in free platelets. The ED50 for ADP (0.15 microM) and for collagen (0.2 microg/mL) was about 1/20 the ED50 found by aggregometry, indicating 20-fold greater sensitivity. (2) At higher concentrations, the fraction of PAg with bound leukocytes (LPAg) increased to 60-70%. This rise correlated with PAg size and CD62P expression, but not with the number of PAg formed. (3) The response of whole blood (WBD) to agonists was qualitatively different from that of platelet-rich plasma (PRP): in WBD the population of CD62P+ PAg was much higher than in PRP and the population of CD62P+ free platelets was much lower. This imp...Continue Reading

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Citations

Jul 1, 2008·Journal of Neuroinflammation·William A SheremataAlireza Minagar
May 29, 2013·Journal of Visualized Experiments : JoVE·Dumizulu L TemboSamuel C Wassmer
Aug 8, 2008·Experimental Psychology·Paul W Foos, Paula Goolkasian
Feb 8, 2003·Hypertension·Richard A PrestonYeon S Ahn
Sep 3, 2009·Neurological Research·Lawrence L HorstmanYeon S Ahn
Nov 1, 2011·Platelets·Stefanie KrajewskiAndreas Straub
Mar 27, 2001·Journal of Biomaterials Science. Polymer Edition·C H Gemmell
May 29, 2004·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Maria Cinta García-MartínezAntonio Cano
Feb 21, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·F L HanebuttR Knoefler
Apr 28, 2012·Blood·Michele P Lambert
Jun 28, 2016·Experimental and Therapeutic Medicine·Shuxia ChenYuping Han
Jul 13, 2017·Oxidative Medicine and Cellular Longevity·Ilaria MarroccoIlaria Peluso
Oct 25, 2000·Circulation·M MertenP Thiagarajan
Jul 15, 2005·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Maike P TiedeYeon S Ahn
Feb 26, 2000·British Journal of Haematology·A H Sutor
Dec 8, 2005·Parasitology Research·Joanna Matowicka-KarnaAnna Butkiewicz
Aug 10, 1999·Critical Reviews in Oncology/hematology·L L Horstman, Y S Ahn

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