Abstract
Heat-killed group A Streptococcus pyogenes induced platelet aggregation in platelet-rich plasma. Aggregation was dependent upon the ratio of platelets to bacteria, with maximal aggregation occurring at 0.8 platelets per bacterium (final concentration, 300,000 per microliter). Inhibition of the reaction by 3 mM EDTA indicated it was a true aggregation and not merely adhesion and agglutination. Lactic acid dehydrogenase assays indicated lysis of platelets did not occur during a 6-min incubation period. Aggregation was inhibited in a dose-dependent manner by acetylsalicylic acid (100 microM to 10 mM) and quinacrine (15.6 to 250 microM), with no decrease in aggregation at the lowest concentration of inhibitor tested. S. pyogenes induced the release of [14C]serotonin, which was maximal (50%) at 2.4 min, when aggregation was nearly complete. Gel-filtered platelets were not aggregated unless fibrinogen (final concentration, 1.8 mg/ml) was included in the reaction mixture. Staphylococcus aureus, a group B streptococcus, and Escherichia coli were unable to induce aggregation in platelet-rich plasma under the conditions used for S. pyogenes.
References
Jan 1, 1979·Infection and Immunity·F J RourkeM S Wilder
May 1, 1977·Infection and Immunity·E H BeacheyA H Kang
Jun 1, 1978·Infection and Immunity·D C MorrisonP M Henson
Oct 1, 1979·The Journal of Clinical Investigation·J HawigerR M Des Prez
Dec 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·T H Spaet, I Lejnieks
Apr 1, 1981·Infection and Immunity·P A Maisch, R A Calderone
Jul 1, 1981·Infection and Immunity·C J Czuprynski, E Balish
Jan 1, 1981·Immunobiology·K H Schmidt, W Köhler
Dec 1, 1981·Infection and Immunity·K G SkerlT Sreevalsan
Jul 1, 1980·The Journal of Infectious Diseases·R M Des PrezJ Hawiger
Jan 1, 1953·American Journal of Clinical Pathology·G BRECHERE P CRONKITE
Aug 1, 1963·The Journal of Physiology·G V BORN, M J CROSS
Jan 1, 1965·The Journal of Pathology and Bacteriology·A W BERNHEIMER, L L SCHWARTZ
May 1, 1965·The Journal of Experimental Medicine·F S KANTOR
Sep 1, 1962·Journal of Clinical Pathology
Citations
Feb 15, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J A Frontera, J D Gradon
Mar 10, 2010·Future Microbiology·Michael R Yeaman
Jan 1, 1991·Microbiology and Immunology·Y UsuiK Yoshida
Mar 31, 2004·Clinical Cardiology·N Semnur BuyukasýkDeniz Demirkan
May 20, 2006·Nature Reviews. Microbiology·J Ross FitzgeraldDermot Cox
Jan 22, 2010·Cellular and Molecular Life Sciences : CMLS·Steven W Kerrigan, Dermot Cox
Jun 18, 2010·Canadian Journal of Physiology and Pharmacology·Archibald McNicol, Sara J Israels
Sep 27, 2019·Current Drug Targets·Victoria A Ploplis, Francis J Castellino
Dec 6, 2011·PloS One·Daniel JohanssonMagnus Rasmussen
Oct 24, 2002·Blood·Ulf SjöbringZaverio M Ruggeri
Jul 10, 2001·Molecular Microbiology·M Rasmussen, L Björck
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Sarbani BhattacharyaFrancis J Castellino
Jul 30, 2014·Infection and Immunity·Lisbeth SvenssonOonagh Shannon
Jan 1, 1991·Microbiology and Immunology·Y UsuiK Yoshida
Nov 23, 2005·Journal of Food Protection·J S ZhouH S Gill
Oct 18, 2000·Infection and Immunity·M RasmussenL Björck
Dec 17, 2009·Cellular and Molecular Life Sciences : CMLS·Michael R Yeaman
Jan 21, 2021·Journal of Thrombosis and Haemostasis : JTH·Samantha G PalaceMilka Koupenova
Nov 14, 1997·Infection and Immunity·A S BayerA L Cheung
Feb 1, 1992·Infection and Immunity·M SimonetP Berche
Aug 1, 1985·Journal of Clinical Microbiology·L J ForresterJ T Barrett
Aug 1, 1987·Infection and Immunity·P M SullamJ Mills