Platelet and endothelial cell P-selectin are required for host defense against Klebsiella pneumoniae-induced pneumosepsis

Journal of Thrombosis and Haemostasis : JTH
Sacha F de StoppelaarTom van der Poll

Abstract

Sepsis is associated with activation of platelets and endothelial cells accompanied by enhanced P-selectin surface expression. Both platelet- and endothelial P-selectin have been associated with leukocyte recruitment and induction of inflammatory alterations. Klebsiella (K.) pneumoniae is a common human sepsis pathogen, particularly in the context of pneumonia. Wild-type (WT) and P-selectin-deficient (Selp(-/-) ) mice or bone marrow chimeric mice were infected with K. pneumoniae via the airways to induce pneumosepsis. Mice were sacrificed during early (12 h after infection) or late-stage (44 h) sepsis for analyses, or followed in a survival study. Selp(-/-) mice displayed 10-1000-fold higher bacterial burdens in the lungs, blood and distant organs during late-stage sepsis. P-selectin deficiency did not influence leukocyte recruitment to the lungs, but was associated with decreased platelet-monocyte complexes and increased cytokine release. Bone marrow transfer studies revealed a role for both platelet and endothelial cell P-selectin as mice deficient in platelet or endothelial cell P-selectin displayed an intermediate phenotype in bacterial loads and survival compared with full wild-type or full knockout control mice. Both plat...Continue Reading

References

Aug 1, 1996·The Journal of Experimental Medicine·J P MizgerdC M Doerschuk
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·P AndréD D Wagner
Nov 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K SingbartlK Ley
Jul 9, 2003·Critical Care Medicine·Sacha ZeerlederC Erik Hack
Mar 23, 2005·The Journal of Experimental Medicine·Ian Del CondeVahid Afshar-Kharghan
Dec 5, 2006·The Journal of Clinical Investigation·Alexander ZarbockKlaus Ley
Nov 23, 2007·Journal of Leukocyte Biology·Marcel SchoutenTom van der Poll
Dec 8, 2007·The Lancet Infectious Diseases·Tom van der Poll, Steven M Opal
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Indira NeeliMarko Radic
May 24, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Stylianos BournazosIan Dransfield
Sep 20, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Kavita N PatelRolando E Rumbaut
Nov 28, 2008·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Alexander Zarbock, Klaus Ley
Oct 12, 2010·Lancet·Neill K J AdhikariGordon D Rubenfeld
Nov 9, 2010·The Journal of Infection·Cheol-In KangUNKNOWN Korean Network for Study of Infectious Diseases (KONSID)
Nov 13, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Renata Polanowska-GrabowskaJoel Linden
Mar 26, 2011·Nature Reviews. Immunology·John W SempleJohn Freedman
Mar 29, 2011·Critical Care Clinics·Marcel LeviTom van der Poll
Mar 28, 2012·The Journal of Experimental Medicine·Marie-Luise von BrühlSteffen Massberg
Aug 21, 2012·The Journal of Infectious Diseases·Miriam H P van LieshoutTom van der Poll
Aug 30, 2013·The New England Journal of Medicine·Derek C Angus, Tom van der Poll
Sep 21, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sif GudbrandsdottirClaus H Nielsen
Oct 18, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jillian StephenIan Dransfield

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Citations

Jul 17, 2015·Journal of Thrombosis and Haemostasis : JTH·S F de StoppelaarT van der Poll
May 18, 2016·American Journal of Respiratory Cell and Molecular Biology·Yanira Riffo-VasquezClive P Page
Mar 16, 2017·PLoS Neglected Tropical Diseases·Emma BirnieW Joost Wiersinga
May 17, 2018·Physiological Reviews·Lee J QuintonJoseph P Mizgerd
Feb 21, 2018·Platelets·Michaela FinsterbuschAlice Assinger
Mar 20, 2018·American Journal of Respiratory Cell and Molecular Biology·Elizabeth A MiddletonGuy A Zimmerman
Jun 23, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Theodora A M ClaushuisTom van der Poll
Sep 12, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Béatrice Hechler, Christian Gachet
Nov 17, 2016·Platelets·Bhanu K ManneMatthew T Rondina
Aug 6, 2019·Frontiers in Immunology·Alice AssingerJulie Rayes
Nov 11, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Chao DingTom van der Poll
Sep 29, 2017·American Journal of Respiratory Cell and Molecular Biology·Richard T AmisonClive P Page
Apr 11, 2019·International Journal of Molecular Sciences·Cristina Gomez-CasadoMaria M Escribese
Jan 29, 2020·Research and Practice in Thrombosis and Haemostasis·Julie RayesSteve P Watson
Jan 24, 2021·The Clinical Respiratory Journal·Sevgen Tanır BasaranogluMehmet Ceyhan
Jun 8, 2021·Frontiers in Immunology·Fatemeh AsgariCecilia Garlanda
Sep 17, 2021·European Respiratory Review : an Official Journal of the European Respiratory Society·Mohamad ChebboDelphine Gras
Dec 18, 2018·Journal of Innate Immunity·Theodora A M ClaushuisTom van der Poll

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