Platelet and Red Blood Cell Counts, as well as the Concentrations of Uric Acid, but Not Homocysteinaemia or Oxidative Stress, Contribute Mostly to Platelet Reactivity in Older Adults

Oxidative Medicine and Cellular Longevity
Kamil KarolczakC Watala

Abstract

The goal of this study was to estimate the hierarchical contribution of the most commonly recognized cardiovascular risk factors associated with atherogenesis to activation and reactivity of blood platelets in a group of men and women at ages 60-65. Socioeconomic and anthropometric data were taken from questionnaires. Blood morphology and biochemistry were measured with standard diagnostic methods. Plasma serum homocysteine was measured by immunochemical method. Plasma concentrations of VCAM, ICAM, total antioxidant status, and total oxidant status were estimated with commercial ELISA kits. Markers of oxidative stress of plasma and platelet proteins (concentrations of protein free thiol and amino groups) and lipids (concentrations of lipid peroxides) and generation of superoxide anion by platelets were measured with colorimetric methods. Platelet reactivity was estimated by impedance aggregometry with arachidonate, collagen, and ADP as agonists. Expression of selectin-P and GPIIb/IIIa on blood platelets was tested by flow cytometry. Platelet aggregation associated significantly negatively with HGB and age and significantly positively with PLT, MPV, PCT, PDW, and P-LCR. When platelet reactivity ("cumulative platelet reactivity_a...Continue Reading

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Citations

Nov 13, 2019·The International Journal of Neuroscience·Shrey PatelSardar Ali Khan
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Marco BernardiAntonio Spataro
Apr 5, 2020·Internal and Emergency Medicine·Arrigo Francesco Giuseppe CiceroClaudio Borghi
Jul 6, 2021·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Wenwei RenJincai He

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Methods Mentioned

BETA
Protein Assay
pharmacotherapy
ELISA
flow cytometry

Software Mentioned

Statistica
StatsDirect
Statsoft
GraphPad Prism
Resampling Stats Add - in for Excel

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