Platelet-derived growth factor accelerates gastric epithelial restoration in a rabbit cultured cell model

Gastroenterology
S WatanabeN Sato

Abstract

Growth factors play an important role in gastric wo und repair. The aim of this study was to assess the role of platelet-derived growth factor (PDGF) in gastric epithelial restoration. PDGF-BB (1-50 ng/mL) was added to confluent cultures of rabbit gastric epithelial cells after wounding. Regrowth of the epithelial cells was monitored for 48 hours. The speed of cell migration was measured, and cell proliferation was detected by using a 5-bromodeoxyuridine (BrdU) staining technique. The labeling index was calculated for a 0.05-mm(2) area around the wound. After wounding, cells at the wound edge formed lamellipodia and showed ruffling movements. The addition of PDGF-BB significantly accelerated cell migration and proliferation as well as gastric restoration. Migration speed was 21 microm/h in control cultures and 32 microm/h and 40 microm/h in cultures containing 10 ng/mL and 50 ng/mL of PDGF-BB, respectively. In control cultures, BrdU-positive cells were rarely detected in the initial 24 hours after wounding, and maximum labeling occurred at 36 hours (labeling index, 3.4%). Cultures containing PDGF-BB showed maximum labeling at 24 hours (labeling index, 6.9%). PDGF-BB dose-dependently accelerated the migration rate and proliferat...Continue Reading

Citations

May 29, 2001·Microscopy Research and Technique·T PohleW Domschke
Oct 24, 2007·The Journal of Surgical Research·Outi Nylander-KoskiHarri Mustonen
May 20, 1998·Clinical and Experimental Pharmacology & Physiology·M D Basson
Mar 30, 2007·Digestive Diseases and Sciences·Masaru OdashimaSumio Watanabe
Apr 6, 2007·Digestive Diseases and Sciences·Kikuko AmagaseKoji Takeuchi
Jun 4, 1999·Journal of Gastroenterology and Hepatology·T OsadaS Watanabe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Zeitschrift für Psychosomatische Medizin und Psychotherapie
Kerstin WeidnerYve Stöbel-Richter
© 2021 Meta ULC. All rights reserved