Platelet-derived growth factor receptor-β (PDGFRβ) lineage tracing highlights perivascular cell to myofibroblast transdifferentiation during post-traumatic osteoarthritis.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Takashi SonoAaron W James

Abstract

Pericytes ubiquitously surround capillaries and microvessels within vascularized tissues and have diverse functions after tissue injury. In addition to regulation of angiogenesis and tissue regeneration after injury, pericytes also contribute to organ fibrosis. Destabilization of the medial meniscus (DMM) phenocopies post-traumatic osteoarthritis, yet little is known regarding the impact of DMM surgery on knee joint-associated pericytes and their cellular descendants. Here, inducible platelet-derived growth factor receptor-β (PDGFRβ)-CreERT2 reporter mice were subjected to DMM surgery, and lineage tracing studies performed over an 8-week period. Results showed that at baseline PDGFRβ reporter activity highlights abluminal perivascular cells within synovial and infrapatellar fat pad (IFP) tissues. DMM induces a temporospatially patterned increase in vascular density within synovial and subsynovial tissues. Marked vasculogenesis within IFP was accompanied by expansion of PDGFRβ reporter+ perivascular cell numbers, detachment of mGFP+ descendants from vessel walls, and aberrant adoption of myofibroblastic markers among mGFP+ cells including α-SMA, ED-A, and TGF-β1. At later timepoints, fibrotic changes and vascular maturation occu...Continue Reading

References

May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Oct 26, 2005·Osteoarthritis and Cartilage·K P H PritzkerW B van den Berg
Feb 13, 2007·Nature Cell Biology·Arianna DellavalleGiulio Cossu
Sep 13, 2008·Cell Stem Cell·Mihaela CrisanBruno Péault
Dec 17, 2009·The American Journal of Pathology·Benjamin D HumphreysJeremy S Duffield
Nov 21, 2012·Stem Cells·Chien-Wen ChenJohnny Huard
Aug 9, 2013·American Journal of Respiratory and Critical Care Medicine·Chi HungJeremy S Duffield
Jul 30, 2014·Methods in Molecular Biology·Julia Lorenz, Susanne Grässel
Dec 4, 2014·Cell Stem Cell·Rafael KramannBenjamin D Humphreys
Oct 20, 2015·ELife·Katharina S VolzKristy Red-Horse
Dec 19, 2015·Cellular and Molecular Life Sciences : CMLS·Ian A DarbyAlexis Desmoulière
May 17, 2017·Nature Communications·Do Young ParkGou Young Koh
Jul 8, 2017·Tissue Engineering. Part a·Carolyn A MeyersAaron W James
Oct 25, 2017·Nature Communications·I R MurrayN C Henderson
Apr 3, 2019·Advances in Experimental Medicine and Biology·Chi F HungLynn M Schnapp

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Citations

Nov 14, 2020·Current Opinion in Rheumatology·Natalia Zapata-LinaresXavier Houard
Mar 30, 2021·Current Tissue Microenvironment Reports·Sanjiv KaulAnusha Mishra

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