Platelet-derived growth factor receptors form complexes with neuropilin-1 during megakaryocytic differentiation of thrombopoietin-dependent UT-7/TPO cells

Biochemical and Biophysical Research Communications
Akimichi OhsakaN Komatsu

Abstract

Neuropilin-1 (NRP-1) is involved in angiogenesis, but the role of NRP-1 in megakaryocytopoiesis is not yet fully understood. In this study, we investigated whether thrombopoietin (TPO) regulates the expression of platelet-derived growth factor (PDGF) and its receptors (PDGFRs) on TPO-dependent UT-7/TPO cells and whether PDGFRs and NRP-1 on UT-7/TPO cells form complexes during megakaryocytic differentiation. When UT-7/TPO cells were starved of TPO for 24 h and then stimulated with 5 ng/ml TPO, the expression of PDGF-B, PDGFRα, and PDGFRβ were significantly up-regulated after the addition of TPO. TPO also induced tyrosine phosphorylation of PDGFRα but not PDGFRβ, and promoted the formation of PDGFRαβ heterodimer complexes. Furthermore, megakaryocytic differentiation of UT-7/TPO cells on treatment with phorbol myristate acetate (PMA) was accompanied by a marked up-regulation of PDGFRβ and NRP-1 protein expression, complex formation between PDGFRs and NRP-1, PDGFRαβ heterodimer complexes, and an increase in PDGF-BB-binding activity. Immunocytochemistry confirmed complex formation between PDGFRs and NRP-1 and PDGFRαβ heterodimer complexes in PMA-differentiated UT-7/TPO cells. Our observations suggest that NRP-1 is involved in megaka...Continue Reading

References

Apr 12, 2001·Genes & Development·A C GingrasN Sonenberg
Feb 14, 2002·Trends in Cardiovascular Medicine·Gera NeufeldYael Herzog
Jun 25, 2002·Genes & Development·Diane C FingarJohn Blenis
Mar 28, 2008·The Biochemical Journal·Caroline Pellet-ManyIan Zachary
May 17, 2008·Genes & Development·Johanna AndraeChrister Betsholtz
Oct 28, 2009·Biochemical and Biophysical Research Communications·Akimichi OhsakaNorio Komatsu
Feb 11, 2011·The Biochemical Journal·Caroline Pellet-ManyIan C Zachary

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Citations

Jan 31, 2019·International Journal of Molecular Sciences·Shira ToledanoGera Neufeld
Feb 6, 2019·International Journal of Molecular Sciences·Stephan Niland, Johannes A Eble
Apr 13, 2018·Biomedicines·Jing Hao, Jennifer S Yu
Jan 4, 2020·Journal of Experimental & Clinical Cancer Research : CR·Enrica VescarelliSimona Ceccarelli

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