Platelet dysfunction in asphyxiated newborn piglets resuscitated with 21% and 100% oxygen

Pediatric Research
Po-Yin CheungM W Radomski

Abstract

Hemostatic disturbances are common in asphyxiated newborns after resuscitation. We compared platelet function in hypoxic newborn piglets reoxygenated with 21% or 100% oxygen. Piglets (1-3 d, 1.5-2.1 kg) were anesthetized and acutely instrumented for hemodynamic monitoring. After stabilization, normocapnic hypoxia was induced with an inspired oxygen concentration of 10-15% for 2 h. Piglets were then resuscitated for 1 h with 21% or 100% oxygen, followed by 3 h with 21% oxygen. Platelet counts and collagen (2, 5, and 10 microg/mL)-stimulated whole blood aggregation were studied before hypoxia and at 4 h of post-hypoxia/reoxygenation. Platelet function was studied using transmission electron microscopy and by measuring plasma thromboxane B2 (TxB2) and matrix metalloproteinase (MMP)-2 and -9 levels. Control piglets were sham-operated without hypoxia/reoxygenation. The hypoxemic (PaO2 33 mm Hg) piglets developed hypotension with metabolic acidosis (pH 7.02-7.05). Upon reoxygenation, piglets recovered and blood gases gradually normalized. At 4 h reoxygenation, platelet aggregation ex vivo was impaired as evidenced by a rightward-downward shifting of the concentration-response curves. Electron microscopy showed features of platelet ac...Continue Reading

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Citations

Feb 1, 2008·Intensive Care Medicine·Po-Yin CheungDavid L Bigam
Oct 19, 2011·Journal of Visualized Experiments : JoVE·Po-Yin CheungDavid L Bigam
Jun 18, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·A SolaV Favareto
Feb 2, 2011·The Journal of Pediatrics·Mary E BaumanM Patricia Massicotte
Jun 1, 2007·Acta Paediatrica·Ola Didrik Saugstad
Jul 14, 2010·European Journal of Pharmacology·Marwan EmaraPo-Yin Cheung
Aug 28, 2007·Archives of Biochemistry and Biophysics·Marwan EmaraPo-Yin Cheung
Jun 6, 2020·Frontiers in Pediatrics·Mary O'DeaEleanor J Molloy
Jun 3, 2021·International Journal of Molecular Sciences·Jakub MocholAdrian Doroszko

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