Platelet hypersensitivity induced by cholesterol incorporation

The Journal of Clinical Investigation
S J ShattilR A Cooper

Abstract

Platelets from individuals with familial hypercholesterolemia show increased sensitivity to the aggregating atents, epinephrine and ADP. Since the mechanism of this abnormal sensitivity is unknown, we examined, in vitro, the influence of the plasma lipid environment on the function of platelets. The composition of plasma lipids was altered by the addition of sonicated cholesterol-dipalmitoyl lecithin liposomes which were "cholesterol normal" (cholesterol-phospholipid mole ratio [C/P] equals 1.0, "cholesterol rich" (C/P eauals 2.2), or "cholesterol poor" (C/P equals 0). Cholesterol-normal liposomes had no influence on platelet lipids or platelet function. In contrast, after incubation for 5 h at 37 degrees C with cholesterol-rich liposomes, normal platelets acquired 39.2% excess cholesterol with no change in phospholipids or protein. The percent increase in platelet membrane cholesterol was three-fold that of the granule fraction. The acquisition of cholesterol by platelets was associated with a 35-fold increase in sensitivity to epinephrine-induced aggregation (P less than 0.001) and 15-fold increase to ADP aggregation (P less than 0.001), as determined both by aggregometry and by [13C]serotonin release. Response to thrombin or...Continue Reading

References

Jan 1, 1966·The Journal of Clinical Investigation·A J MarcusH L Ullman
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Feb 21, 1974·The New England Journal of Medicine·A C CarvalhoR S Lees
Jun 6, 1974·The New England Journal of Medicine·R A CooperJ R Durocher
Oct 1, 1969·The Journal of Clinical Investigation·R A Cooper
Mar 1, 1967·Clinica Chimica Acta; International Journal of Clinical Chemistry·W H FishmanS Green
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE
Oct 1, 1951·Proceedings of the Society for Experimental Biology and Medicine·J S HAGERMAN, R G GOULD

❮ Previous
Next ❯

Citations

Jul 1, 1991·American Journal of Hematology·M MillerP Kwiterovich
Dec 1, 1992·Clinical Cardiology·F E KuhnC E Rackley
Jul 1, 1992·Journal of Clinical Pharmacology·J FuchsI Weinberger
Jan 25, 2002·Journal of Cellular Physiology·Karine GoussetFern Tablin
Jan 1, 1984·Biulleten' eksperimental'noĭ biologii i meditsiny·E A GorbatenkovaV B Razumov
Jan 1, 1991·European Journal of Clinical Pharmacology·A BergJ Keul
Jan 1, 1980·The Journal of Membrane Biology·R D SauerheberL M Gordon
Sep 1, 1986·Basic Research in Cardiology·J NimpfT Kenner
Sep 1, 2006·Digestive Diseases and Sciences·Vinay SundaramStephen H Caldwell
Jun 7, 2013·Pharmaceutical Research·Sounik SarkarAnjan Kr Dasgupta
Jun 23, 1981·Biochimica Et Biophysica Acta·L A SchulerJ F Strauss
Feb 8, 1985·Biochimica Et Biophysica Acta·B P Schick, P K Schick
Dec 15, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·C LechiA Lechi
Dec 31, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·N Chetty, N H Naran
Oct 14, 1985·Life Sciences·B M Cohen, G S Zubenko
Jan 1, 1993·Life Sciences·N VlasicM B Stemerman
Jul 1, 1979·Progress in Cardiovascular Diseases·A I Schafer, R I Handin
Sep 1, 1983·Thrombosis Research·T SanoH Yamazaki
Jan 1, 1978·Progress in Lipid Research·F P Bell
Jan 1, 1981·Progress in Lipid Research·D G Cornwell, R V Panganamala
Oct 1, 1994·Psychiatry Research·J K YaoJ L Peters
Mar 1, 1987·Prostaglandins, Leukotrienes, and Medicine·R J BarnardM A Kirschenbaum

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.