Platelet lysate supports the in vitro expansion of human periodontal ligament stem cells for cytotherapeutic use

Journal of Tissue Engineering and Regenerative Medicine
Rui-Xin WuFa-Ming Chen

Abstract

Human platelet lysate (PL) produced under optimal conditions of standardization and safety has been increasingly suggested as the future 'gold standard' supplement to replace fetal bovine serum (FBS) for the ex vivo propagation of mesenchymal stem cells for translational medicine and cell therapy applications. However, the multifaceted effects of PL on tissue-specific stem cells remain largely unexplored. In the present study, we investigated the stem cell behaviours of human periodontal ligament stem cells (PDLSCs) in media with or without PL. Our data indicate that human PL, either as an adjuvant for culture media or as a substitute for FBS, supports the proliferation and expansion of human PDLSCs derived from either 'young' or 'old' donors to the same extent as FBS, without interfering with their immunomodulatory capacities. Although PL appears to inhibit the in vitro differentiation of 'young' or 'old' PDLSCs, their decreased osteogenic potential may be restored to similar or higher levels compared with FBS-expanded cells. PL- and FBS-expanded PDLSCs exhibited a similar potential to form mineralized nodules and expressed similar levels of osteogenic genes. Our data indicate that large clinically relevant quantities of PDLSC...Continue Reading

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Citations

Jul 13, 2016·Journal of Tissue Engineering and Regenerative Medicine·Fen LiuFa-Ming Chen
Jul 18, 2017·Journal of Tissue Engineering and Regenerative Medicine·Bei-Min TianFa-Ming Chen
Jun 16, 2018·Journal of Materials Science. Materials in Medicine·Leopoldina D F AlmeidaManuela E Gomes
Sep 13, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yan GaoHao Chen
May 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pedro S BaboManuela E Gomes
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Siddharth ShanbhagKamal Mustafa
Jul 10, 2017·ACS Biomaterials Science & Engineering·Pedro S BaboManuela E Gomes

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