Platelet lysates from aged donors promote human tenocyte proliferation and migration in a concentration-dependent manner

Bone & Joint Research
D R BergerN J Steinmetz

Abstract

Platelet-rich plasma (PRP) is being used increasingly often in the clinical setting to treat tendon-related pathologies. Yet the optimal PRP preparations to promote tendon healing in different patient populations are poorly defined. Here, we sought to determine whether increasing the concentration of platelet-derived proteins within a derivative of PRP, platelet lysate (PL), enhances tenocyte proliferation and migration in vitro, and whether the mitogenic properties of PL change with donor age. Concentrated PLs from both young (< 50 years) and aged (> 50 years) donors were prepared by exposing pooled PRP to a series of freeze-thaw cycles followed by dilution in plasma, and the levels of several platelet-derived proteins were measured using multiplex immunoassay technology. Human tenocytes were cultured with PLs to simulate a clinically relevant PRP treatment range, and cell growth and migration were assessed using DNA quantitation and gap closure assays, respectively. Platelet-derived protein levels increased alongside higher PL concentrations, and PLs from both age groups improved tenocyte proliferation relative to control conditions. However, PLs from aged donors yielded a dose-response relationship in tenocyte behaviour, wit...Continue Reading

References

Jul 31, 2003·Cell·Dong-Wan SeoWilliam G Stetler-Stevenson
Apr 6, 2006·Clinical Oral Implants Research·Filippo GrazianiMario Gabriele
Sep 23, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Chia-Wen HsuChuen-Chyi Tseng
Oct 9, 2012·Clinics in Sports Medicine·Robert Z Tashjian
Jun 12, 2013·Frontiers in Bioscience (Elite Edition)·Geoffroy NourissatFrancis Berenbaum
Jul 22, 2014·Trends in Biotechnology·Kyriakos SpanoudesDimitrios I Zeugolis
Aug 26, 2014·BioMed Research International·Ilaria GiustiVincenza Dolo
Dec 3, 2014·Advanced Drug Delivery Reviews·Denitsa DochevaChristopher H Evans
Jan 14, 2016·BMC Musculoskeletal Disorders·Iris Sophie AlbersInge Van den Akker-Scheek
Mar 18, 2016·Bone & Joint Research·I R Murray, R F LaPrade
Feb 10, 2017·Transfusion·Gabriel StrandbergDavid Berglund
Feb 18, 2017·Orthopaedic Journal of Sports Medicine·Joanne Y ZhangKristofer J Jones
Jan 5, 2018·Expert Opinion on Biological Therapy·Isabel AndiaNicola Maffulli
Mar 31, 2018·Clinical Orthopaedics and Related Research·Yi-Jun ZhangXiang-Jin Lin

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Citations

Dec 1, 2020·Platelets·Paula Oneto, Julia Etulain
Dec 1, 2020·Platelets·Lassina BarroThierry Burnouf
Oct 27, 2020·Bone & Joint Research·Miquel Antich-RossellóJoana M Ramis
Aug 14, 2021·Journal of Clinical Orthopaedics and Trauma·Lucas da FonsecaJosé Fábio Lana
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Anna Lange-ConsiglioFausto Cremonesi

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Methods Mentioned

BETA
DNA assay

Software Mentioned

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ImageJ
GraphPad Prism

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