Abstract
Platelet-rich plasma (PRP) is being used increasingly often in the clinical setting to treat tendon-related pathologies. Yet the optimal PRP preparations to promote tendon healing in different patient populations are poorly defined. Here, we sought to determine whether increasing the concentration of platelet-derived proteins within a derivative of PRP, platelet lysate (PL), enhances tenocyte proliferation and migration in vitro, and whether the mitogenic properties of PL change with donor age. Concentrated PLs from both young (< 50 years) and aged (> 50 years) donors were prepared by exposing pooled PRP to a series of freeze-thaw cycles followed by dilution in plasma, and the levels of several platelet-derived proteins were measured using multiplex immunoassay technology. Human tenocytes were cultured with PLs to simulate a clinically relevant PRP treatment range, and cell growth and migration were assessed using DNA quantitation and gap closure assays, respectively. Platelet-derived protein levels increased alongside higher PL concentrations, and PLs from both age groups improved tenocyte proliferation relative to control conditions. However, PLs from aged donors yielded a dose-response relationship in tenocyte behaviour, wit...Continue Reading
References
Nov 14, 1997·British Journal of Haematology·T MurateT Hayakawa
Jul 31, 2003·Cell·Dong-Wan SeoWilliam G Stetler-Stevenson
Apr 6, 2006·Clinical Oral Implants Research·Filippo GrazianiMario Gabriele
Sep 23, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Chia-Wen HsuChuen-Chyi Tseng
Jul 23, 2011·The Korean journal of laboratory medicine·Hee Soon ChoKyung Eun Song
Sep 29, 2011·Tissue Engineering. Part C, Methods·Jun ArakiKotaro Yoshimura
Mar 1, 2012·The American Journal of Sports Medicine·Chris Hyunchul JoSue Shin
Oct 9, 2012·Clinics in Sports Medicine·Robert Z Tashjian
Jun 12, 2013·Frontiers in Bioscience (Elite Edition)·Geoffroy NourissatFrancis Berenbaum
Jun 14, 2013·Stem Cell Research & Therapy·Paola Romina AmableRadovan Borojevic
Feb 25, 2014·British Journal of Sports Medicine·Robert-Jan de VosAdam Weir
Jul 22, 2014·Trends in Biotechnology·Kyriakos SpanoudesDimitrios I Zeugolis
Aug 26, 2014·BioMed Research International·Ilaria GiustiVincenza Dolo
Dec 3, 2014·Advanced Drug Delivery Reviews·Denitsa DochevaChristopher H Evans
Dec 24, 2015·Frontiers in Aging Neuroscience·Vincenzo SaliniMichele Abate
Jan 14, 2016·BMC Musculoskeletal Disorders·Iris Sophie AlbersInge Van den Akker-Scheek
Feb 11, 2016·Bone & Joint Research·Y H RohJ H Oh
Mar 18, 2016·Bone & Joint Research·I R Murray, R F LaPrade
Jun 9, 2016·The American Journal of Sports Medicine·Jane FitzpatrickMing H Zheng
Feb 10, 2017·Transfusion·Gabriel StrandbergDavid Berglund
Feb 18, 2017·Orthopaedic Journal of Sports Medicine·Joanne Y ZhangKristofer J Jones
Nov 28, 2017·BMJ Open Sport & Exercise Medicine·Larry E MillerSamir Bhattacharyya
Dec 22, 2017·The American Journal of Sports Medicine·Xiao ChenC Thomas Vangsness
Jan 5, 2018·Expert Opinion on Biological Therapy·Isabel AndiaNicola Maffulli
Jan 26, 2018·Scientific Reports·Julia EtulainMirta Schattner
Mar 31, 2018·Clinical Orthopaedics and Related Research·Yi-Jun ZhangXiang-Jin Lin
Citations
Aug 14, 2020·International Journal of Molecular Sciences·Pietro Gentile, Simone Garcovich
Nov 27, 2020·PeerJ·Olav K Straum
Dec 1, 2020·Platelets·Paula Oneto, Julia Etulain
Dec 1, 2020·Platelets·Lassina BarroThierry Burnouf
Oct 27, 2020·Bone & Joint Research·Miquel Antich-RossellóJoana M Ramis
Aug 14, 2021·Journal of Clinical Orthopaedics and Trauma·Lucas da FonsecaJosé Fábio Lana
Aug 17, 2021·The American Journal of Sports Medicine·Dustin R BergerNeven J Steinmetz
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Anna Lange-ConsiglioFausto Cremonesi
Oct 12, 2021·Bone & Joint Research·Anita Sanghani-KeraiNoel Fitzpatrick